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目的观察ＨＰＶ１６、１８早期蛋白Ｅ６在人宫颈鳞状细胞癌中的表达并评价Ｅ６单抗的应用价值。方法采用ＳＰ免疫组化染色法，检测４０例宫颈鳞状细胞癌，３０例慢性宫颈炎及３０例正常宫颈组织中ＨＰＶ１６、１８Ｅ６蛋白的表达。结果癌组中Ｅ６的阳性率为６７．５％（２７／４０），慢性宫颈炎中为３．３％（１／３０），正常宫颈组织中均为阴性，良、恶组ＨＰＶ１６、１８Ｅ６的阳性率差异有显著意义（Ｐ＜０．０１）。结论ＨＰＶ１６、１８感染与本地区宫颈癌病因学密切相关，Ｅ６单抗可作为预测ＨＰＶ１６、１８感染及宫颈癌早期诊断的标记之一。
Objective To observe the expression of HPV16,18 early protein E6 in human cervical squamous cell carcinoma and evaluate the value of E6 monoclonal antibody. Methods SP immunohistochemical staining was used to detect the expression of HPV16, 18E6 protein in 40 cases of cervical squamous cell carcinoma, 30 cases of chronic cervicitis and 30 cases of normal cervical tissue. Results The positive rate of E6 was 67.5% (27/40) in cancer group and 3.3% (1/30) in chronic cervicitis, which was negative in normal cervical tissue. The positive rates of HPV16, 18E6 The positive rate difference was significant (P <0.01). Conclusion HPV16,18 infection is closely related to the etiology of cervical cancer in our region. E6 monoclonal antibody can be used as one of the markers to predict HPV16,18 infection and early diagnosis of cervical cancer.