论文部分内容阅读
对兔血浆及组织器官中钙拮抗剂尼群地平的分析方法进行了研究，建立了反相高效液相色谱（RP-HPLC）的内标测定法。样品在弱碱性条件下经环已烷-异丙醇（96．5：3．5）提取，用YWGC18反相柱，以地尔硫作内标，甲醇：50mmol／L磷酸盐缓冲液（70：30）pH6．8为流动相，UV236nm检测。方法最低检测限约为10ng，血药浓度在21．6～216μg／L之间，尼群地平的量与其峰面积比值之间，线性关系良好，回归方程为Y＝0．00672X－0．013，r＝0．9958。方法回收率95．4％～98．1％，RSD＜4．63％。 用该分析方法测定了家兔口服尼群地平2h后在主要脏器体液中的分布。
The analytical method of nitrendipine, a calcium antagonist in the plasma and tissues and organs of rabbits, was studied. The internal standard method of RP-HPLC was established. Samples were extracted with cyclohexane-isopropanol (96.5: 3.5) in weakly basic conditions using a reverse-phase YWGC18 column with diltiazem as internal standard and methanol: 50 mmol / L phosphate buffer ( 70:30) pH6.8 as mobile phase, UV236nm detection. The minimum detection limit was 10 ng and the plasma concentration was between 21.6 and 216 μg / L. The linear relationship between the amount of nitrendipine and its peak area ratio was good. The regression equation was Y = 0.00672X-0.013 , R = 0.9958. The recovery rate was 95.4% ~ 98.1% with RSD <4.63%. Using this method, the distribution of nitrendipine in rabbits’ body fluids after 2h oral administration was determined.