论文部分内容阅读
目的采用高效液相色谱法(HPLC)同时测定宽筋藤药材中盐酸药根碱、盐酸假巴马汀和盐酸巴马汀三种成分的含量。方法使用OMNI C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-2.5 mmo L/L庚烷磺酸钠和50 mmo L/L磷酸二氢钾(含三乙胺0.14%)(22:78);检测波长345 nm;柱温40℃;流速1.0 m L/min。结果盐酸药根碱、盐酸假巴马汀和盐酸巴马汀分别在1.872~56.16μg/m L、8.37~167.4μg/m L、2.014~60.42μg/m L浓度范围内具有良好的线性;平均加样回收率依次为:102.19%(RSD=1.70%)、102.89%(RSD=1.09%)、102.38%(RSD=1.17%)。结论建立的分析方法准确、可靠,重复性好,可用于宽筋藤药材的质量控制。
Objective To determine the contents of jatrorrhizine hydrochloride, pseudomephedrine hydrochloride and palmatine hydrochloride in Radix Glabra by high performance liquid chromatography (HPLC). Methods OMNI C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-2.5 mmo L / L heptane sulfonate and 50 mmo L / L potassium dihydrogen phosphate with 0.14% triethylamine 22:78); detection wavelength 345 nm; column temperature 40 ℃; flow rate 1.0 m L / min. Results The pharmacokinetics of jatrorrhizine hydrochloride, palmatine hydrochloride and palmatine hydrochloride showed good linearity in the range of 1.872-56.16 μg / mL, 8.37-167.4 μg / mL and 2.014-60.42 μg / mL, respectively. The mean The recoveries of samples were 102.19% (RSD = 1.70%), 102.89% (RSD = 1.09%) and 102.38% (RSD = 1.17%). Conclusion The established analytical method is accurate, reliable and reproducible. It can be used for quality control of Radix.