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二甲戊乐灵是农业生产中使用量较大的一种芽前二硝基苯胺除草剂,其在环境中的持久性已经引起广泛关注。为更好地理解其在厌氧条件下的环境归趋,文章研究了Shewanella marisflavi EP1厌氧生物转化二甲戊乐灵的能力以及不同环境因子对该转化的影响。试验以乳酸钠作为电子供体,二甲戊乐灵为唯一的电子受体,EP1为还原菌,分别考察不同的反应体系和条件下EP1的生长及其对二甲戊乐灵还原的情况。结果表明,S.marisflavi EP1在厌氧条件下能有效还原转化二甲戊乐灵,核黄素的存在加快了还原反应速率,而呼吸抑制剂能够延迟甚至终止还原转化反应,表明二甲戊乐灵的还原转化是与电子传递链相关联的微生物呼吸过程。此外,EP1还原二甲戊乐灵的最优条件分别为p H 8,温度35℃,盐度2%。该研究不仅扩展了希瓦氏菌利用电子受体的潜力,也为处理硝基苯胺类农药的污染修复提供依据。
Pendimethalin is a pre-emergent dinitroaniline herbicide which is widely used in agricultural production. Its persistence in the environment has drawn wide attention. In order to better understand its environmental fate under anaerobic conditions, the ability of Shewanella marisflavi EP1 to anaerobically convert pendimethalin and the effects of different environmental factors on this transformation were studied. In the experiment, sodium lactate was used as the electron donor, pendimethalin was the only electron acceptor and EP1 was the reducing bacteria. The growth of EP1 and the reduction of pendimethalin were investigated under different reaction systems and conditions respectively. The results showed that S.marisflavi EP1 could effectively reduce and convert pendimethalin under anaerobic conditions, and the presence of riboflavin accelerated the reduction reaction rate, whereas the respiratory inhibitor could delay or even terminate the conversion reaction, indicating that pendimethalin Spiritual reduction transformation is the microbial respiration process associated with the electron transport chain. In addition, the optimal conditions for the reduction of pendimethalin by EP1 are p H 8, respectively, at a temperature of 35 ° C and a salinity of 2%. This study not only extends the potential of Shewanella to utilize electron acceptors, but also provides a basis for dealing with the pollution remediation of nitroaniline pesticides.