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在盐度为12的海水中,添加不同浓度的CaCl2(2、4、6、8、10g/L)可对中华绒螯蟹肝胰腺中超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、过氧化氢酶(CAT)以及溶菌酶(LZM)的活性产生影响。结果表明:在实验海水中浸泡4h后,实验组与对照组河蟹肝胰腺中5种酶的活性随着CaCl2浓度的上升均出现了不同程度的降低,当CaCl2浓度为10g/L时,活性最低;ACP和AKP分别为对照组的56.72%和40.76%;SOD与CAT的活性在10g/L时为对照组的20.11%和7.32%;LZM的活性为对照组的60.14%;该结果说明:正常海水中CaCl2浓度的提高会导致河蟹体内自由基代谢的紊乱,从而影响机体的正常生理功能和免疫力。除LZM外,当CaCl2浓度为4g/L时,其它免疫酶的活性均表现出向下的拐点。因此,继续提高海水中的CaCl2浓度,将对河蟹成活率产生不利的影响。
In the salinity of 12 seawater, adding different concentrations of CaCl2 (2,4,6,8,10 g / L) in liver and pancreas of Eriocheir sinensis superoxide dismutase (SOD), alkaline phosphatase (AKP ), ACP, CAT and LZM. The results showed that the activities of five enzymes in hepatopancreas of experimental group and control group decreased with the increase of CaCl2 concentration after immersing in experimental seawater for 4h, and the lowest activity was found when CaCl2 concentration was 10g / L ; ACP and AKP were 56.72% and 40.76% of the control group respectively; the activities of SOD and CAT were 20.11% and 7.32% of the control group at 10g / L; the activity of LZM was 60.14% of the control group; The increase of CaCl2 concentration in seawater will lead to the disorder of free radical metabolism in crab body, which will affect the body’s normal physiological function and immunity. With the exception of LZM, all other immune enzymes showed a downward turning point when the CaCl2 concentration was 4 g / L. Therefore, continuing to raise the concentration of CaCl2 in seawater will adversely affect the survival rate of crabs.