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利用手术切除阴茎包皮 ,剪碎后经胰蛋白酶消化 ,添加 16 40培养液和表皮生长因子 ,制成细胞悬液 ,接种于垫有塑料薄膜载体的培养瓶中 ,经体外培养和传代培养 ,可在载体上得到大面积的表皮细胞生长薄膜 ,在移植时可将载体从培养瓶中取出 ,使长有表皮细胞薄膜一面贴敷于深二度烧伤创面和肉芽创面 ,一周后 ,可见移植创面长满培养的表皮细胞。经大量临床治疗证明 ,有很好的疗效。因此 ,以阴茎包皮作为培养皮肤细胞来源 ,通过增殖不仅达到扩大表皮面积目的 ,而且能较快地修复创面 ,有很好的实用价值和发展前景。
Surgical removal of the penicillin, after cutting and tryptic digestion, adding 1640 culture medium and epidermal growth factor, made of cell suspension, inoculated in plastic flasks with culture media carrier, by in vitro culture and subculture, can A large area of the epidermal cell growth membrane was obtained on the carrier. During the transplantation, the carrier could be removed from the culture flask, and the epidermal cell membrane with long epidermis cell membrane was applied to the second-degree burns and granulation wounds. After one week, Full of cultured epidermal cells. After a large number of clinical treatment proved that there is a good effect. Therefore, the use of the penis foreskin as a source of skin cells, not only through the proliferation of epidermal area to expand the purpose, but also to quickly repair the wound, have good practical value and development prospects.