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目的:探讨DNA双链断裂修复蛋白RAD51在喉癌细胞中的表达及其临床意义。方法:应用免疫组织化学SP法检测喉癌和声带息肉组织中RAD51的表达情况,并结合肿瘤的临床TNM分期、淋巴结转移及其病理分化程度等分析它在肿瘤发生发展中的作用。结果:①RAD51在喉癌组中的表达明显强于声带息肉组,差异有统计学意义(P<0.01);②RAD51在喉癌组3~4期中的表达强于1~2期喉癌,差异有统计学意义(P<0.01);③RAD51在低、中分化喉癌中的表达强于高分化喉癌(P<0.05),差异有统计学意义;④RAD51在有淋巴结转移喉癌中的表达强于无淋巴结转移喉癌,差异有统计学意义(P<0.05)。结论:RAD51在喉癌的发生、发展中可能起重要作用,它可能成为临床诊断和治疗喉癌的一个潜在靶点,对预测肿瘤的预后有一定的意义。
Objective: To investigate the expression of DNA double-strand break repair protein RAD51 in laryngeal carcinoma cells and its clinical significance. Methods: The expression of RAD51 in laryngeal carcinoma and vocal cord polyps was detected by immunohistochemical SP method. The expression of RAD51 was analyzed in combination with the clinical TNM stage, lymph node metastasis and pathological differentiation. Results: ①RAD51 expression in laryngeal carcinoma was significantly higher than that in vocal cord polyps, the difference was statistically significant (P <0.01); ②RAD51 expression in laryngeal carcinoma stage 3-4 was stronger than that in laryngeal carcinoma of stage 1-2, the difference was (P <0.01). The expression of RAD51 in low and moderately differentiated laryngeal carcinoma was stronger than that in well differentiated laryngeal carcinoma (P <0.05), and the difference was statistically significant. The expression of RAD51 in laryngeal carcinoma with lymph node metastasis was stronger than that in laryngeal carcinoma No lymph node metastasis of laryngeal cancer, the difference was statistically significant (P <0.05). Conclusion: RAD51 may play an important role in the occurrence and development of laryngeal cancer. It may become a potential target for the clinical diagnosis and treatment of laryngeal cancer, which is of certain significance in predicting the prognosis of cancer.