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以大丽轮枝菌(Verticillium dahliae Kleb.)菌体为唯一碳源,采用液体培养法测定供试病原菌菌体对4株拮抗链霉菌胞壁水解酶合成的诱导作用;采用平皿气生菌丝水解和显微观察法验证4株拮抗链霉菌对大丽轮枝菌菌丝的溶解作用。结果表明:①大丽轮枝菌菌体可以诱导供试链霉菌合成几丁质酶、β-1,3-葡聚糖酶、β-葡萄糖苷酶及滤纸纤维素酶;当菌体添加量为10 g.L-1,28℃培养7 d时,上述4种诱导酶活性可达峰值,其值分别为70.13-129.05、31.04-37.34、6.24-8.75及1.84-3.35 U。②链霉菌粗酶液对大丽轮枝菌菌丝有溶解作用。③4株链霉菌菌丝均可缠绕在大丽轮枝菌菌丝上,并在缠绕处使病原菌菌丝溶解。
Verticillium dahliae Kleb. Was used as the sole carbon source. Liquid culture was used to determine the induction of cell wall hydrolyzing enzyme in four Streptomyces strains against pathogenic bacteria. Hydrolysis and microscopic observation of four Streptomyces antagonistic strains of V. dahliae lysis. The results showed that: (1) Verticillium dahliae could induce the synthesis of chitinase, β-1,3-glucanase, β-glucosidase and filter paper cellulase from Streptomyces testes; When cultured at 10 gL-1,28 ℃ for 7 d, the above-mentioned four kinds of inducible enzymes reached the peak values, which were 70.13-129.05, 31.04-37.34, 6.24-8.75 and 1.84-3.35 U, respectively. ② Streptomyces crude enzyme solution of V. dahliae mycelium have a dissolving effect. ③ 4 strains of Streptomyces mycelium can be wound on the mycelium of Verticillium dahlia, and wound in the pathogen mycelium dissolved.