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作者对前人在稀酸条件下使胺苯硫脲(Thiace-tazone)水解,再经重氮化并与α-萘乙烯二胺偶合后的比色测定法作了改进,即在碱性水解条件下,使胺苯硫脲完全水解,水解产物再经重氮化后显色,进行比色测定。本法的优点在于水解完全,使最终产物经显色后可于550毫微米处呈现一尖锐的吸收峰;而在酸性条件下水解由于可能混杂着未完全水解的产物,因而吸收峰变宽,本法特别适用于含有异烟肼的
The authors made improvements to the thiace-tazone hydrolyzed by thiacetyl thiourea (urea) under the condition of dilute acid, and then improved by colorimetry after diazotization and coupling with α-Naphthalene ethylenediamine, Under the conditions, the amine phenylthiourea completely hydrolyzed hydrolysates diazotization after color, colorimetric determination. The advantage of this method is that the hydrolysis is complete and the final product shows a sharp absorption peak at 550 nm after color development. Under acidic conditions, the hydrolysis may broaden the absorption peak due to the possibility of mixing with incompletely hydrolyzed product, This Law is particularly applicable to containing isoniazid