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把外源性DNA引入活细胞的转染技术大大地促进了病毒分子生物学的研究。目前应用最广泛的转染技术是由磷酸钙和DEAE-dextran介导的,最近又建立了一种新的脂质体转染技术。然面,所有这些方法都有耗时、繁琐和昂贵等缺点。应用24孔和98孔细胞培养板的新的小型转染技术解决了这些问题,并在不同的细胞系的病毒基因的即时转染和稳定转染中获得成功。作者在人横纹肌肉瘤(RD),神经纤维瘤(SK-N-MC)和Hela细胞系上,用带HIV LT
Transfection techniques that introduce exogenous DNA into living cells have greatly facilitated the study of viral molecular biology. Currently the most widely used transfection technology is mediated by calcium phosphate and DEAE-dextran, and recently a new liposome transfection technique was established. However, all of these methods are time consuming, tedious and expensive. The new mini-transfection technology using 24-well and 98-well cell culture plates solved these problems and succeeded in both immediate and stable transfection of viral genes in different cell lines. The authors performed on human rhabdomyosarcoma (RD), neurofibroma (SK-N-MC) and Hela cell lines with HIV LT