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目的:探讨糖槭多糖对S_(180)荷瘤小鼠的抑瘤率及其红细胞免疫调节机制,以此来评价糖槭多糖对红细胞的免疫作用。方法:以S_(180)肿瘤模型小鼠为体内实验对象研究不同剂量糖槭多糖(145、72.5、36.25 mg/kg)的抑瘤率;采用比色法测定红细胞唾液酸(SA)含量、抗氧化酶系的活力;流式细胞术检测荷瘤小鼠红细胞C_(3b)数量,红细胞免疫花环实验检测荷瘤小鼠红细胞C_(3b)受体的活性。结果:与阴性对照组相比,不同剂量多糖抑瘤率均在25%以上,其中高剂量组(145 mg/kg)效果最好,达到41.24%;提高S_(180)荷瘤小鼠红细胞唾液酸(SA)含量和抗氧化酶系的活性;提高荷瘤小鼠红细胞C_(3b)受体的数量和活性。结论:糖槭多糖能明显抑制肿瘤生长;通过提高S_(180)荷瘤小鼠SA和抗氧化酶系活性,进而提高C_(3b)受体的活性和数量,是糖槭多糖抗肿瘤作用的免疫机制之一。
OBJECTIVE: To investigate the inhibitory rate of polysaccharide from polysaccharides from Maple and Maple on S 180 tumor-bearing mice and its mechanism of erythrocyte immunoregulation in order to evaluate its immunogenicity on erythrocytes. Methods: The antitumour rates of polysaccharides (145, 72.5, 36.25 mg / kg) of polysaccharides of Maple Leaf were studied in S 180 tumor model mice. The contents of erythrocyte sialic acid (SA) Oxidase activity. The number of erythrocytes C_ (3b) in tumor-bearing mice was detected by flow cytometry. The activity of erythrocyte C_ (3b) receptor in tumor-bearing mice was detected by erythrocyte immune rosette assay. Results: Compared with the negative control group, the inhibitory rates of polysaccharides of different dosages were more than 25%, of which the high dose group (145 mg / kg) had the best effect (41.24%), and the erythrocyte salivary Acid (SA) content and antioxidant enzyme activity; increase the number and activity of erythrocyte C_ (3b) receptors in tumor-bearing mice. CONCLUSION: Polysaccharides from Maple Leaf can inhibit tumor growth significantly. Antitumor effect of polysaccharide from Maple Leaf is enhanced by increasing SA and antioxidant enzyme activity of S_ (180) tumor-bearing mice and further increasing the activity and quantity of C_ (3b) receptor One of the immune mechanisms.