论文部分内容阅读
目的 探讨CD30阳性的窦性大B细胞淋巴瘤 (SLBCL)的临床病理学特征及其与Epstei Barr(EB)病毒的关系。方法 结合临床特点、形态学及免疫表型分析 ,并通过聚合酶链反应 (PCR)法检测EB病毒BamHIWDNA片段及免疫组织化学法检测潜伏膜蛋白 1(LMP1) ,对 2例CD30阳性的SLBCL进行分析。结果 2例分别为 6 4岁男性及 85岁女性 ,均表现为浅表淋巴结肿大 ,其中 1例 10个月后死于肿瘤。镜下 ,肿瘤都以窦性分布且彼此黏附的生长方式及相对单形的细胞形态为特征。免疫表型分析提示 ,两者均呈CD4 5、CD30、CD2 0或CD79α阳性而不表达上皮膜抗原 (EMA)、ALK1及组织细胞或T细胞的标志。未发现 2例有EB病毒感染的证据。结论 CD30阳性的SLBCL是一种形态、表型独特的弥漫大B细胞淋巴瘤变异类型 ,该瘤需与T/裸细胞性间变性大细胞淋巴瘤及其他一些累及淋巴窦为主的淋巴结病变相鉴别 ,但与EB病毒可能无关。
Objective To investigate the clinicopathological features of CD30-positive sinusoidal B-cell lymphoma (SLBCL) and its relationship with Epstein Barr (EB) virus. Methods According to clinical features, morphological and immunophenotypic analysis, BamHIW DNA fragment of Epstein-Barr virus (EBV) was detected by polymerase chain reaction (PCR) and LMP1 was detected by immunohistochemistry. Two cases of CD30-positive SLBCL analysis. Results Two cases were male aged 64 and female aged 85 respectively. All showed superficial lymph nodes, of which 1 died of tumor after 10 months. Microscopically, the tumors were characterized by a sinusoidally distributed and adherent growth pattern and a relatively monomorphic cell morphology. Immunophenotypic analysis suggested that both were hallmarks of CD4 5, CD30, CD20 or CD79α positive but not epithelial membrane antigen (EMA), ALK1, and tissue or T cells. No evidence of EBV infection was found in 2 cases. Conclusions CD30-positive SLBCL is a type of diffuse large B-cell lymphoma with a unique phenotype. This tumor needs to be associated with T / idiopathic large cell lymphoma and other lymphadenopathy involving lymphoid sinus Identification, but may not have Epstein-Barr virus.