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异色瓢虫Harmonia axyridis(Pallas)在亚洲地区是一种重要的捕食性天敌昆虫,但是当引入到欧美后,对当地的物种产生了威胁。本文用ISSR技术对两个中国本地种群和一个引入美国的种群进行了种群多样性以及种群分化的分析。8条ISSR引物共扩增出105条带,其中多态性条带比率为96.19%。Nei’s多样性指数、Shannon信息指数、多态性条带比率,在中国种群与美国种群中均非常接近,种群分化不明显(G_(st)=0.042)。AMOVA分析显示种群多样性主要来源于种群内部,种群间的基因流非常强(N_m=5.537)。基于Nei’s多样性构建的聚类结果显示ISSR是一种快速有效的研究异色瓢虫种群多样性关系的技术。
Harmonia axyridis (Pallas), an important predator of insects in the Asian region, poses a threat to local species when introduced into Europe and the United States. This article uses ISSR technology to analyze the population diversity and population differentiation of two Chinese indigenous populations and one introduced into the United States. A total of 105 bands were amplified by 8 ISSR primers, of which the percentage of polymorphic bands was 96.19%. The Nei’s diversity index, Shannon’s information index and polymorphic band ratio were very similar between Chinese and American populations, and the population differentiation was not obvious (G st = 0.042). The AMOVA analysis showed that the population diversity mainly came from the inside of the population, and the gene flow among the populations was very strong (Nm = 5.537). The clustering results based on Nei’s diversity show that ISSR is a rapid and effective technique for studying the relationship between populations of Harmonia axyridis populations.