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我所于1979年8月开始进行杉木离体组织再生植株的试验研究,至11月25日已陆续培养出一批杉木试管苗.现将结果初报如下:一、材料与方法取1—2年生的杉木苗,去叶,流水冲洗后用滤纸吸干.接种前先用75%酒精处理1—2秒钟,再放入0.1%氯化汞中消毒8—10分钟,用无菌水冲洗4—5次.在无菌条件下将嫩茎切成0.5—1.0厘米大小的切段,接种到培养基上.培养基采用MS、1/2MS为基本培养基.分化培养基蔗糖含量为3%,生根培养基蔗糖含量为1%,琼脂均为0.8%.所有培养基PH均调至5.8,并经1.1公斤/厘米~2热压消毒15分钟.
I started in 1979 August fir tissue regeneration of plant tissue research, to November 25 has been to train a group of fir plantlets. The results are as follows: First, the material and method to take 1-2 The annual seedlings of Fir, to the leaves, washed with water filter paper blotted dry before inoculation with 75% alcohol for 1-2 seconds, and then placed in 0.1% mercuric chloride disinfection 8-10 minutes, rinsed with sterile water 4-5 times under aseptic conditions will cut the stems cut into 0.5-1.0 cm size sections and inoculated into the medium using MS, 1 / 2MS as the basic medium differentiation medium sucrose content of 3 %, Rooting medium sucrose content of 1%, agar 0.8%, all medium PH were adjusted to 5.8, and 1.1 kg / cm ~ 2 autoclave 15 minutes.