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目的检测壳寡糖对人肝癌SMMC-7721细胞的抑制效果及对凋亡调控蛋白Bcl-2和Caspase-3的影响。方法采用噻唑蓝(MTT)法检测不同浓度壳寡糖对肝癌细胞SMMC-7721细胞增殖的抑制作用,并利用荧光Hoechst33258染色法检测细胞凋亡状况。最后通过免疫细胞化学方法研究壳寡糖对肝癌细胞SMMC-7721中Bcl-2和Caspase-3表达的影响。结果壳寡糖能够抑制SMMC-7721细胞增殖,并且促进SMMC-7721细胞的凋亡,并且壳寡糖能够上调促凋亡蛋白Caspase-3的表达和降低抑制凋亡蛋白Bcl-2的表达。结论壳寡糖对人肝癌SMMC-7721细胞的增殖有抑制作用,此作用可能是通过促进Caspase-3的表达和抑制Bcl-2的表达来实现的。
Objective To detect the inhibitory effect of chitooligosaccharides on human hepatocellular carcinoma SMMC-7721 cells and its effect on apoptosis regulatory proteins Bcl-2 and Caspase-3. Methods MTT assay was used to detect the inhibitory effect of different concentrations of chitooligosaccharides on the proliferation of SMMC-7721 cells. Fluorescence Hoechst 33258 staining was used to detect the apoptosis. Finally, the effects of oligochitosan on the expression of Bcl-2 and Caspase-3 in hepatocellular carcinoma cell line SMMC-7721 were studied by immunocytochemistry. Results Chitosan oligosaccharide could inhibit the proliferation of SMMC-7721 cells and promote the apoptosis of SMMC-7721 cells. Chitosan oligosaccharide could up-regulate the expression of Caspase-3 and decrease the expression of Bcl-2. Conclusion Chitooligosaccharides can inhibit the proliferation of human hepatocellular carcinoma SMMC-7721 cells, and this effect may be through promoting the expression of Caspase-3 and inhibiting the expression of Bcl-2.