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采用未改性硅胶作固定相的高效液相色谱法同时测定复方黄连素注射液中的硫酸氢小檗碱和甲氧苄氨嘧啶(TMP)的含量。实验表明,以甲醇-2%三乙胺水溶液(磷调节pH至7.0)(80∶20)作为流动相,得到满意的分离效果。该两组分的回收率分别为100.8%和99.9%,RSD均不大于1.0%(n=5)。
Simultaneous determination of berberine and trimethoprim (TMP) in compound berberine injection by high performance liquid chromatography with unmodified silica as stationary phase. Experiments showed that the methanol--2% triethylamine aqueous solution (phosphorus adjusted to pH 7.0) (80:20) was used as the mobile phase to obtain a satisfactory separation effect. The recoveries of the two components were 100.8% and 99.9%, respectively, and the RSD was not greater than 1.0% (n=5).