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丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)能提高植物抗重金属毒性。为评价镉(Cd)胁迫条件下AMF对烟草生长和抗Cd毒性的影响,对烟苗(Nicotiana tabaccum L.)进行0、5、20、40 mg/kg Cd处理,接种和不接种AMF玫瑰红巨孢囊霉Gigaspora rosea的双因子试验。培养90 d后测定植株的营养生长量、渗透调节物质和叶片保护酶活性。0~5 mg/kg Cd处理下,无论接种或不接种AMF,烟草的营养生长、渗透调节物质含量、叶片保护酶活性都上升。5 mg/kg Cd处理下,接种与不接种AMF的烟草各项指标均达到各处理的最高值,并显著高于0 mg/kg的对照组;Cd浓度继续升高时,烟草的营养生长、渗透调节物质含量、叶片保护酶活性均下降。在0~40 mg/kg Cd浓度处理范围内,各项检测指标的总体趋势是先升后降;在相同Cd浓度处理中,接种AMF烟草,其营养生长、渗透调节物质含量和叶片保护酶活性均高于不接种的烟草。结果表明:低于或等于5 mg/kg Cd浓度处理,能促进烟草的营养生长和抗重金镉毒性能力的提高,高于5 mg/kg Cd浓度处理时,Cd对烟草植株产生毒害,且随着浓度的升高毒性加重,用AMF Gigaspora rosea接种烟苗,可以减轻烟苗受Cd胁迫的毒性。
Arbuscular mycorrhizal fungi (AMF) can enhance the plant’s resistance to heavy metal toxicity. To evaluate the effects of AMF on tobacco growth and Cd toxicity under Cd stress, Nicotiana tabacum L. was treated with 0, 5, 20, and 40 mg / kg Cd, with or without AMF Rose Bengal Two-factor test of Gigaspora rosea. After 90 days of culture, the vegetative growth, the osmoregulation substances and the protective enzyme activities of leaves were measured. At 0 ~ 5 mg / kg Cd treatment, the vegetative growth, osmolyte content and the protective enzyme activity of tobacco increased with or without AMF inoculation. Under the treatment of 5 mg / kg Cd, the indicators of tobacco with or without AMF inoculated with and without AMF reached the highest value of each treatment and were significantly higher than that of 0 mg / kg control group. When the Cd concentration continued to increase, the nutrition growth, Osmotic adjustment material content, leaf protective enzyme activity decreased. In the range of 0-40 mg / kg Cd treatment, the overall trend of all the indexes was first increased and then decreased. At the same Cd concentration, AMF tobacco inoculated with AMF tobacco had the effects of nutrient growth, osmotic adjustment substance content and leaf protective enzyme activity Are higher than the non-inoculated tobacco. The results showed that Cd treatment at or below 5 mg / kg could promote the vegetative growth and anti-Cd-Cd toxicity of tobacco, and Cd toxicity to tobacco plants was higher than that of Cd treatment at 5 mg / kg Cd With the increase of concentration, the toxicity is aggravated. Inoculation of tobacco seedlings with AMF Gigaspora rosea can reduce the toxicity of tobacco seedlings to Cd stress.