为了探索更加方便快捷的鉴定花生杂种子代的方法,本研究以花优7号和花优4号为亲本,根据其SNP位点的突变碱基G-T和SSR多态性位点,同时利用籽仁表型性状的观察、SSR多态性标记分析、四引物扩增突变体系PCR和Sanger测序基因峰值图分析等四种方法,进行F1代真伪杂种鉴定.结果表明,根据籽仁的表型性状来鉴别杂种F1的真伪,只能鉴别出部分杂种,有一定局限性;SSR多态性分子标记法需要多对引物并进行多次筛选,该方法比较简单,成熟,适用于大群体;ARMS-PCR方法则仅需2对引物;PCR扩增产物的鉴定只需要普通的琼脂糖凝胶电泳即可,省时省力,简便,但其对引物设计要求比较高;基因序列峰值图分析较耗时耗力耗财,适用于小群体.因此,运用后三种生物技术方法均能鉴别F1代真伪杂种,F1代真杂种的准确鉴定可以减少F2群体的规模,有助于进一步的遗传群体构建和新品种的培育.“,”In order to find a more convenient and quick way to distinguish the hybrid in peanut cross breeding,Huayou 7 and Huayou 4 were selected as parents.According to its SNP locus mutation based on G-T and SSR polymorphism loci,at the same time using the four methods of observing the seed ofphenotypic traits,SSR marker analysis,tetra-primer amplification refractor mutation system PCR and Sanger sequence peak analysis,the authenticity was determined in F1 hybrids.The results showed that observing the phenotypic traits of seed only could ide'ntify some parts of the hybrid,which had certain limitation.SSR polymorphic molecular markers need many pairs of primers,multiple screening and simple,mature,suitable for large groups.ARMS-PCR only need 2 pairs of primers.PCR amplification results only need agarose gel,so it could save time and effort,and was simple and convenient,but the primer requirements were relatively high.The Sanger sequence peak value analysis of gene sequence was more time-consuming and resource consuming,suitable for small groups.Therefore,these later three biotechnology methods could be used to identify F1 generation hybrids.The accurate identification of F1 true hybrids could reduce the size of the F2 population,and it might be beneficial to the further construction of genetic population and the breeding of new varieties.