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用成年猫单侧备用根模型 (切断一侧 L1～ 5 、L7～ S2 节段脊髓背根 ,保留 L6 背根 ) ,用免疫组织化学和原位杂交技术 ,对脊髓背角 板层、背核和 L6 背根节中神经生长因子的表达进行了动态观察。结果证明 ,部分背根去传入后 :(1)脊髓各节段双侧背角 板层的神经生长因子和神经生长因子 m RNA阳性神经元的数量及体积显著增高、增大 ;(2 )手术侧背核神经生长因子和神经生长因子 m RNA阳性神经元的增高强于非手术侧 ,但术后两时间组的手术侧之间无差异 ;(3 )备用根背根节新出现了神经生长因子 m RNA阳性神经元 ,且神经生长因子阳性神经元数量明显高于正常组 ,且随术后时间增加而递增。以上结果表明 :部分背根去传入猫脊髓背角 板层及背核内神经生长因子水平提高 ,尤其备用根背根节神经元神经生长因子的增加 ,为备用背根传入纤维侧支生芽和突触重建提供了良好的条件 ,有助于解释脊髓初级传入纤维损伤溃变后代偿性机能恢复的机制
The unilateral lateral root model of adult cats (cut off the L1 ~ 5, L7 ~ S2 segments of the dorsal root of the spinal cord and retain the L6 dorsal root) with immunohistochemistry and in situ hybridization, spinal dorsal horn, dorsal nucleus And the expression of nerve growth factor in L6 DRG were observed dynamically. The results showed that after some dorsal roots were introduced, (1) the number and volume of nerve growth factor and nerve growth factor m RNA positive neurons in the bilateral dorsal horn in each segment of spinal cord were significantly increased and increased; (2) The increase of nerve growth factor and nerve growth factor m RNA positive neurons in the dorsal radicular nucleus of the dorsal radicular was stronger than that of the non-surgical dorsal horn, but no difference was found between the two sides of the operation time. (3) Growth factor m RNA positive neurons, and the number of nerve growth factor positive neurons was significantly higher than the normal group, and increased with time after the increase. The above results show that: some dorsal roots into the cat litter spinal dorsal horn and dorsal raphe increased levels of nerve growth factor, especially in the dorsal root ganglion neurons increase in nerve growth factor, the dorsal root for the introduction of fiber lateral branch Buds and synaptic reconstruction provide good conditions to help explain the mechanism of compensatory functional recovery after primary afferent fiber injury in the spinal cord