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目的 探讨孕妇不同血清HBV DNA含量对HBV母婴传播的影响。方法 应用荧光定量PCR技术检测 6 9对母婴血清HBV DNA含量 (所测数据经对数转换 ) ,对孕妇不同血清HBV DNA含量进行分组。结果 孕妇HBV DNA含量为 (6 3± 1 9)拷贝 /ml,婴儿为 (4 8± 2 0 )拷贝 /ml,两者之间呈正相关 (r=0 310 ,P <0 0 1)。 6 9例婴儿中 ,45例HBV DNA定性和 (或 )HBV血清学检查异常 ,提示HBV母婴垂直传播 ,传播率为 6 5 %;HBV母婴传播率随孕妇血清HBV DNA浓度增高而增高 ;根据孕妇血清HBV DNA含量分别向下和向上累计分析 ,显示孕妇HBV DNA含量在 5 0、6 0和 7 0拷贝 /ml三个界面的上下浓度区域 ,HBV母婴传播率差异有显著性 ,其差值分别为 32 %、34 %和 2 8%。本组资料中 ,孕妇HBeAg阳性 19例 ,HBsAb阳性 17例 ,HBeAg阳性组HBV DNA[(7 6± 1 3)拷贝 /ml]明显高于阴性组 [(5 8± 1 9)拷贝 /ml],其HBV母婴传播率 (90 %)明显高于阴性组 (5 6 %) ;HBsAb阳性组HBV DNA含量 [(5 3± 1 6 )拷贝 /ml]明显低于阴性组 [(6 6± 1 9)拷贝 /ml],HBV母婴传播率 (2 9%)也明显低于阴性组 (77%)。同时 ,HBeAg阳性主要分布在HBV DNA测定值较高的孕妇中 ,HbsAb阳性主要分布在HBV DNA测定值较低的孕妇组。结论 HBV母婴传播率受孕妇血清H
Objective To investigate the effect of different serum HBV DNA levels in pregnant women on the transmission of HBV in mother and infant. Methods Fluorescent quantitative PCR was used to detect the serum levels of HBV DNA in 69 maternal and infant serum samples. The serum HBV DNA levels of pregnant women were divided into groups. Results HBV DNA level in pregnant women was (63 ± 1 9) copies / ml, and infants was (48 ± 20) copies / ml. There was a positive correlation between HBV DNA levels (r = 0 310, P 0 01). Of the 69 infants, 45 cases of HBV DNA were qualitatively and / or HBV serologically abnormal, which suggested that the mother-to-child transmission of HBV was 65%. The mother-to-child transmission rate of HBV increased with the increase of HBV DNA in pregnant women. According to the accumulative and downward analysis of HBV DNA in pregnant women, the HBV DNA content of pregnant women was in the upper and lower concentration regions of 50, 60 and 70 copies / ml, and there was significant difference in the rate of mother-to-child transmission of HBV The difference is 32%, 34% and 28% respectively. In this study, 19 HBeAg positive pregnant women and 17 HBsAb positive pregnant women were significantly higher than those in the negative group [(58 ± 19) copies / ml] , The HBV-to-HBV transmission rate (90%) was significantly higher than that in the negative group (56%). The HBV DNA level in HBsAb-positive group was significantly lower than that in the negative group [(53 ± 16) copies / 1 9) copies / ml]. The rate of mother-to-child transmission of HBV (29%) was also significantly lower than that of the negative group (77%). At the same time, HBeAg positive mainly in pregnant women with higher HBV DNA, HbsAb positive mainly in pregnant women with lower HBV DNA. Conclusion The rate of mother to child transmission of HBV is affected by serum H