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目的比较女性宫颈正常细胞与不典型鳞状细胞(atypical squamous cells of undetermined significance,ASC-US)中人乳头瘤病毒(human papillomavirus,HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1 000例宫颈正常细胞和229例宫颈ASC-US标本进行23种HPV基因分型检测。结果 1 000例宫颈正常细胞中检出HPV阳性感染者106例,总的HPV感染率为10.60%(106/1 000),其一重感染率为9.30%(93/1 000),多重感染率为1.30%(13/1 000);229例宫颈ASC-US中检出HPV阳性感染者116例,总的HPV感染率为50.66%(116/229),其一重感染率为34.06%(78/229),多重感染率为16.59%(38/229)。两组HPV总阳性率、一重和多重感染率比较差异有统计学意义(P<0.05)。结论 HPV16、18、33、42、43、52、58型是宫颈正常细胞及ASC-US中最主要的型别,基因扩增结合基因芯片检测技术可应用于宫颈细胞的HPV分型检测,有利于在临床上对宫颈ASC-US行进一步分流管理,对宫颈癌的防控具有重要意义。
Objective To compare the genotypes of human papillomavirus (HPV) infection between female normal cervical cells and atypical squamous cells of undetermined significance (ASC-US) and its clinical significance. Methods Twenty-three HPV genotypes were detected in 1 000 cervical normal cells and 229 cervical ASC-US specimens by gene amplification combined with gene chip technique. Results One hundred and sixty-six cases of HPV positive were detected in normal cervical cells. The total HPV infection rate was 10.60% (106/1 000). The rate of one-case HPV infection was 9.30% (93/1 000). The multiple infection rate was 1.30% (13/1 000). Of the 229 HPV-positive cases detected in ASC-US, 116 cases were HPV-positive, with a total HPV infection rate of 50.66% (116/229) and a single-case infection rate of 34.06% (78/229 ), The multiple infection rate was 16.59% (38/229). There was significant difference between the two groups (P <0.05). Conclusion HPV16,18,33,42,43,52,58 is the most common type in cervical normal cells and ASC-US. Gene amplification combined with gene chip detection can be applied to detect HPV types in cervical cells. There are Which is conducive to the further clinical management of cervical ASC-US diversion management, prevention and control of cervical cancer is of great significance.