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目的研究Decapty1/HMG/HCG长方案促超排卵对子宫内膜ICAM-1表达的影响,分析ICAM-1变化与血清E2、P水平之间的相关性,探讨该方案高获卵率而低妊娠率的原因,为临床改善促排卵方案,提高IVF-ET的妊娠率提供理论依据。方法将小白鼠分为NC组和COH组,用免疫组化SP法观察两组小鼠pd1~pd6子宫内膜ICAM-1蛋白的表达。用罗氏电化学发光法检测两组小鼠pd1~pd6血清E2、P的水平。结果NC组中ICAM-1于pd4表达最明显(P(0.01),COH组中ICAM-1于pd3表达最明显(P(0.01)。ICAM-1蛋白的表达高峰和血清高峰点P/E2呈正相关关系(r=0.756,P(0.01)。结论Decapty1/HMG/HCG可能通过改变血清E2、P水平,使子宫内膜种植窗开放异常,导致子宫内膜发育与胚胎发育不同步,降低子宫内膜接受性,这可能是影响IVF-ET妊娠率的重要原因。
Objective To investigate the effect of Decapty1 / HMG / HCG regimen on the expression of ICAM-1 in endometrium and to analyze the correlation between the changes of ICAM-1 and serum E2 and P levels. Rate of reasons for the clinical improvement of ovulation program to improve the pregnancy rate of IVF-ET provide a theoretical basis. Methods The mice were divided into NC group and COH group. The expression of ICAM-1 protein in pd1 ~ pd6 endometrium was detected by immunohistochemical SP method. The levels of E2 and P in pd1 ~ pd6 of two groups of mice were detected by Roche electrochemiluminescence. Results The expression of ICAM-1 in NC group was the highest (P (0.01)), the expression of ICAM-1 in COH group was the highest (P (0.01)), the expression peak of ICAM-1 protein and peak serum P / (R = 0.756, P <0.01) .Conclusion Decapty1 / HMG / HCG may make the endometrial implantation window abnormal by changing the level of serum E2 and P, leading to the asynchronous development of endometrium and embryo development, Membrane receptivity, which may be an important factor affecting the pregnancy rate of IVF-ET.