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辐射损伤修复抑制剂咖啡因(Caf)和Na2EDTA(EDTA)后处理对不同辐射敏感性作物大豆和油菜幼苗超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响呈现为较一致的变化规律。两种作物幼苗下胚轴内,SOD酶活性均随着辐照剂量增大而增大,出现峰值后又陡然降低。大豆幼苗CAT酶活性与SOD酶有大致相同的变化规律,而油菜CAT酶活性在随辐照剂量变化过程中,在300~1000Gy范围内波动较小。POD酶活性则均是随着辐照剂量的增大而增大。除个别处理、个别剂量外,Caf、EDTA后处理与水处理(对照)相比,3种酶的活性有不同程度的提高,说明Caf、EDTA在抑制遗传物质DNA损伤修复的同时,减轻了因辐射产生的自由基造成的毒害。各处理中,抗辐射的油菜幼苗下胚轴中SOD、CAT和POD酶活性都高于对辐射敏感的大豆。
Radiation damage repair inhibitors caffeine and Na2EDTA (EDTA) post-treatment had significant effect on superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity showed a more consistent pattern of changes. In the hypocotyls of both crop seedlings, SOD activity increased with the increase of radiation dose, and then decreased sharply after the peak appeared. Soybean seedling CAT activity and SOD enzyme changes roughly the same, and CAT activity of rapeseed with the irradiation dose changes in the range of 300 ~ 1000Gy less fluctuation. POD activity was increased with the increase of radiation dose. Except for individual treatment and individual dose, Caf, EDTA post-treatment compared with water treatment (control), the activity of the three kinds of enzymes increased to some extent, indicating that Caf, EDTA in the inhibition of genetic material DNA damage repair at the same time, Radiation generated by the free radicals poisoning. In each treatment, the activities of SOD, CAT and POD in radiation-resistant rapeseed hypocotyls were higher than those of radiation-sensitive soybean.