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目的:研究内皮素(endothlin,ET)对人肺成纤维细胞(HLF)、大鼠肝贮脂细胞(Ito细胞)增殖活性和胶原合成的影响及维拉帕米(Ver)对ET促细胞增殖效应的阻断作用.方法:采用胶原酶灌流、密度梯度离心方法分离大鼠肝Ito细胞,并以~3H—TdR、~3H-L-脯氨酸掺入法观察细胞增殖效应.结果:ET对HLF、ItO细胞具有显著的促进DNA合成和细胞增殖作用,并随ET浓度的升高而作用增强.Ver在不抑制细胞DNA合成的剂量范围内,即可拮抗ET的促细胞增殖及胶原合成作用.结论:ET对肝纤维化相关细胞HLE、Ito细胞增殖活性及胶原合成具有促进作用,Ver对ET促细胞增殖效应具有阻断作用.
Objective: To investigate the effect of endothlin (ET) on the proliferation and collagen synthesis of human lung fibroblasts (HLF) and rat hepatocytes (Ito cells) and the effect of verapamil on the proliferation of ET Effect.Methods: Rat liver Ito cells were isolated by collagenase perfusion and density gradient centrifugation, and the effects of 3H-TdR and 3H-proline incorporation were observed.Results: ET ItO cells significantly promote the DNA synthesis and cell proliferation of HLF, and with the role of ET concentration increased.Ver do not inhibit the dose of DNA synthesis in the range, you can antagonize the ET cell proliferation and collagen synthesis .Conclusion: ET can promote the proliferation activity and collagen synthesis of HLE, Ito cells in hepatic fibrosis-related cells, and Ver has a blocking effect on ET-induced cell proliferation.