论文部分内容阅读
作者采用Ｏ型血人新鲜胰腺冰冻切片作抗原，建立了血清胰岛细胞胞浆抗体（ＩＣＡ）的间接免疫荧光测定方法。对１５７例糖尿病患者（其中ＩＤＤＭ８２例、ＮＩＤＤＭ７５例）和８４例正常人进行了血清ＩＣＡ检测。结果，ＩＤＤＭ组、ＮＩＤＤＭ组和对照组的ＩＣＡ阳性率分别为３１．５％、１３．３％和１．１％，３组间差异有非常显著性（Ｐ＜０．００５）。ＩＤＤＭ组中，病程６个月以内者ＩＣＡ阳性率为４１．７％，病程超过６个月者为２２．６％，差异无显著性。１０例ＩＣＡ阳性的ＮＩＤＤＭ病人中，４例为口服降糖药继发失效者。提示ＩＣＡ是ＩＤＤＭ的自身免疫血清学标志，对糖尿病的病因学诊断分型及判断ＮＩＤＤＭ口服降糖药继发失效有重要意义。
The authors used O blood type fresh frozen sections of the pancreas as antigen, established a method of indirect immunofluorescence assay of serum islet cell cytoplasmic antibodies (ICA). 157 patients with diabetes (including IDDM82 cases, NIDDM75 cases) and 84 normal subjects were tested for serum ICA. Results The positive rates of ICA in IDDM group, NIDDM group and control group were 31.5%, 13.3% and 1.1%, respectively. The differences among the three groups were significant (P <0.005). In the IDDM group, the ICA positive rate was 41.7% within 6 months and 22.6% over 6 months, with no significant difference. Of the 10 ICA-positive NIDDM patients, 4 were those who failed secondary oral hypoglycemic agents. It is suggested that ICA is an autoimmune serological marker of IDDM. It is of great significance to diagnose the type of diabetes and determine the secondary failure of NIDDM oral hypoglycemic agents.