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目的:用反相高效液相色谱法同时测定湿生扁蕾中木犀草素、当药黄素和当药醇苷的含量。方法:色谱柱:Agilent SB-C18(250 mm×4.6 mm,5μm);流动相:在0~20 min内,甲醇∶水(含0.04%H3PO4)由40∶60~63∶37线性梯度洗脱,在20~50 min内,甲醇∶水(含0.04%H3PO4)=63∶37等度洗脱;流速:0.8 m L/min;检测波长:254 nm,柱温:30℃。结果:木犀草素在30 min内达到基线分离,回归方程:Y=84 800X+4540(r=0.999 9),线性范围0.848 0~4.240μg;当药黄素在15 min内达到基线分离,回归方程:Y=52 300X-32 300(r=0.999 9),线性范围0.896 0~4.480μg;当药醇苷在20 min内达到基线分离,回归方程:Y=102 000X+83 400(r=0.999 9),线性范围为0.832 0~4.160μg。结论:该测定方法快速,测定结果准确可靠。
Objective: Simultaneous determination of luteolin, daunorubicin and cortisol in wet-growing platyculae by reversed-phase high performance liquid chromatography. Methods: The column was Agilent SB-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol: water (0.04% H3PO4) eluted with a linear gradient from 40:60 to 63:37 within 0-20 min , Methanol: water (0.04% H3PO4) = 63:37 isocratic elution within 20 ~ 50 min; flow rate: 0.8 m L / min; detection wavelength: 254 nm; Results: The luteolin achieved baseline separation within 30 min. The regression equation was Y = 84 800X + 4540 (r = 0.999 9) with a linear range of 0.848 0-4.240 μg. When lutein reached baseline separation within 15 min, regression The equation was: Y = 52 300X-32 300 (r = 0.999 9) with a linear range of 0.896 0-4.480 μg. When the elixir attained baseline separation within 20 min, the regression equation was Y = 102 000X + 83 400 (r = 0.999 9), the linear range of 0.832 0 ~ 4.160μg. Conclusion: The method is rapid and the results are accurate and reliable.