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目的建立同时测定健脾解毒方中原儿茶酸、毛蕊异黄酮葡萄糖苷、人参皂苷Re、人参皂苷Rb1、芹菜素、异甘草素6种成分的UPLC-MS/MS分析方法,并对健脾解毒方中上述成分进行检测。方法采用液质联用方法,应用Poroshell 120 SB-Aq(50 mm×2.1 mm,1.7μm)色谱柱,流动相为0.1%甲酸的水溶液和0.1%乙腈溶液梯度洗脱,采用电喷雾电离源(ESI),多反应离子监测(MRM)扫描方式进行检测。结果健脾解毒方中6种成分线性关系、精密度、稳定性、重复性均符合方法学测定要求,加样回收率为95.2%~102.6%。利用建立的方法测定了6批次健脾解毒方中原儿茶酸等6个成分含量。结论利用建立的方法同时测定健脾解毒方中6种成分的方法具有操作简单、重复性好、准确可靠,可为健脾解毒方质量控制提供依据。
OBJECTIVE To establish a UPLC-MS / MS method for the simultaneous determination of 6 components of protocatechuic acid, calycosin glucoside, ginsenoside Re, ginsenoside Rb1, apigenin and isoliquiritigenin in Jianpi Jiedu Decoction. The above components were tested. Methods A liquid chromatography-mass spectrometry (LC-MS) was performed on a Poroshell 120 SB-Aq (50 mm × 2.1 mm, 1.7 μm) column using a mobile phase of 0.1% formic acid in water and a gradient of 0.1% acetonitrile. ESI), multi-reactive ion monitoring (MRM) scanning mode. The results of Jianpi Jiedu Fang of the six components of the linear relationship, precision, stability, repeatability are in line with the methodological requirements, sample recovery was 95.2% ~ 102.6%. The established method was used to determine the contents of 6 components of protocatechuic acid in 6 batches of Jianpi Jiedu Fang. Conclusions The method of simultaneous determination of six components in Jianpi Jiedu decoction has the advantages of simple operation, good repeatability, accurate and reliable, and can provide basis for quality control of Jianpi Jiedu decoction.