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Aim:To examine the effect of daidzin,genistin,and glycitin on the osteogenic andadipogenic differentiation of bone marrow stromal cells(MSC)and the adipogenictransdifferentiation of osteoblasts.Methods:MTT test,alkaline phosphatase(ALP)activity measurement,Oil Red O stain and measurement were employed.Results:Daidzin,genistin,and glycitin 1×10~(-8),5×10~(-7),1×10~(-6),5×10~(-6),and 1×10~(-5)mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts.Daidzin 5×10~(-7)mol/L and genistin 1×10~(-6)mol/L promoted the osteogenesis ofMSC.Genistin 1×10~(-8),5×10~(-7),1×10~(-6),5×10~(-6),and 1×10~(-5)mol/L and glycitin 1×10~(-8),1×10~(-6),and 1×10~(-5)mol/L inhibited the adipogenesis of MSC.Daidzin,genistin,and glycitin 1×10~(-8),5×10~(-7),1×10~(-6),5×10~(-6),and 1×10~(-5)mol/L all inhibited the adipocytictransdifferentiation of osteoblasts.Conclusions:Daidzin,genistin,and glycitinmay modulate differentiation of MSC to cause a lineage shift toward the osteo-blast and away from the adipocytes,and could inhibit adipocytic transdifferen-tiation of osteoblasts.They could also be helpful in preventing the developmentof osteonecrosis.
Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic metastasis of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. Results: Daidzin, genistin, and glycitin 1 × 10 -8, 5 × 10 -7, 1 × 10 -6, 5 × 10 -6, and 1 Promoted the proliferation of primary mouse bone MSC and osteoblasts.Daidzin 5 × 10 ~ (-7) mol / L and genistin 1 × 10 ~ (-6) mol / L promoted the osteogenesis ofMSC.Genistin 1 × 10 ~ (-8), 5 × 10 ~ (-7), 1 × 10 ~ (-6), 5 × 10 ~ (-6), and 1 × 10 ~ (-5) mol / L and glycitin 1 × 10 ~ (-8), 1 × 10 ~ (-6), and 1 × 10 ~ (-5) mol / L inhibited the adipogenesis of MSC.Daidzin, genistin, and glycitin 1 × 10 ~ -8, 5 × 10 -7, 1 × 10 -6, 5 × 10 -6, and 1 × 10 -5 mol / L all inhibited the adipocytic transmission of osteoblasts. Conclusions: Daidzin, genistin, and glycitinmay modulate differentiation of MSC to cause a lineage shift toward the oste o-blast and away from the adipocytes, and could inhibit adipocytic transdifferen-tiation of osteoblasts. they could also be helpful in preventing the developmentof osteonecrosis.