目的：分离抗人卵巢癌单克隆抗体ＣＯＣ１８３Ｂ２重链可变区（ＶＨ）基因并测定其序列。方法：用反转录ＰＣＲ扩增抗人卵巢癌单抗ＣＯＣ１８３Ｂ２ＶＨ基因，将其克隆入ＰＵＣ１９载体，重组子用Ｓａｎｇｅｒ’ｓ双脱氧链终止法测定序列，将序列与ＧｅｎｅＢａｎｋ及已发表的抗体序列比较。结果：ＶＨ基因全长３５４ｂｐ，属鼠免疫球蛋白重链混杂亚类（ｓｕｂｇｒｏｕｐｍｉｓｃｅｌａｎｅｏｕｓ），由种系基因的ＶＨ，Ｄｓｐ２．５及ＭＵＳＪＨ４重排而来。该ＶＨ基因序列已被ＧｅｎｅＢａｎｋ收录（ａｃｃｅｓｓｉｏｎＮｏＡＦ０４５０２３）。结论：该ＶＨ基因为抗人卵巢癌单抗ＣＯＣ１８３Ｂ２功能性重链可变区基因。 Objective: To isolate the COC183B2 heavy chain variable region (VH) gene of anti-human ovarian cancer monoclonal antibody and determine its sequence. METHODS: The anti-human ovarian cancer monoclonal antibody COC183B2VH gene was amplified by reverse transcriptionPCR and cloned into PUC19 vector. The recombinant was sequenced by Sanger’s dideoxy chain termination method. The sequence was compared with GeneBank and published Antibody sequence comparison. Results: The VH gene was 354 bp in length and belongs to a subgroup of mouse immunoglobulin heavy chain heterozygous rearranged by VH, Dsp2.5 and MUSJH4 of germline genes. The VH gene sequence has been included in GeneBank (acces  developmentNoAF045023). Conclusion: The VH gene is an anti-human ovarian cancer monoclonal antibody COC183  B2 functional heavy chain variable region genes.