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AIM:To investigate gene expression pattern of human γ-synuclein gene in human esophageal squamous cellcarcinoma(ESCC)by using semi-quantitive reversetranscription polymerase chain reaction(RT-PCR),and to studythe role of γ-synuclein in the development of human ESCC.METHODS:Semi-quantitive RT-PCR of 27 pairs ofspecimens of human ESCC tissues and corresponding normaltissues was used to investigate the expression pattern of γ-synuclein in ESCC.9706/γ-syn cells in which γ-synuclein wasoverexpressed were obtained through cloning γ-synucleingene by PCR and transfecting it into ESCC 9706 cells,thenselecting with G-418 for 14 days.The biological effects of y-synuclein were measured and compared between 9706/γ-synand 9706/vec cells by cell growth curve and soft agar assay.RESULTS:RT-PCR showed that γ-synuclein gene wasexpressed in all the 27 cases of normal epithelial tissues,while downregulation of γ-synuclein was observed in 16 outof the 27 cases(59.3 %)of ESCC.There were also 6 casesof ESCC tissues with a high expression level of γ-synucleinmRNA.In functional analysis we found that over-expressionof γ-synuclein in ESCC 9706 cells could inhibit the growthrate and transformation ability of ESCC 9706 cells.CONCLUSION:The low expression level of γ-synuclein inhuman ESCC and the biological effects of γ-synuclein over-expression on ESCC 9706 cells suggest that γ-synucleinmay play a role as a negative regulator in the developmentof human ESCC.
AIM: To investigate gene expression pattern of human γ-synuclein gene in human esophageal squamous cell carcinoma (ESCC) by using semi-quantitive reversetranscription polymerase chain reaction (RT-PCR), and to study the role of γ-synuclein in the development of human ESCC . METHODS: Semi-quantitive RT-PCR of 27 pairs of specific tumors of human ESCC tissues and corresponding normal tissues was used to investigate the expression pattern of γ-synuclein in ESCC.9706 / γ-syn cells in which γ-synuclein wasoverexpressed were obtained through cloning γ-synucleingene by PCR and transfecting it into ESCC 9706 cells, then selecting with G-418 for 14 days. The biological effects of y-synuclein were measured and compared between 9706 / γ-syn and 9706 / vec cells by cell growth curve and soft agar assay .RESULTS: RT-PCR showed that γ-synuclein gene wasexpressed in all the 27 cases of normal epithelial tissues, while downregulation of γ-synuclein was observed in 16 out of the 27 cases (59.3%) of ESCC.There were also 6 cases sof ESCC tissues with a high expression level of γ-synuclein mRNA. In functional analysis we found that over-expression of γ-synuclein in ESCC 9706 cells could inhibit the growthrate and transformation ability of ESCC 9706 cells.CONCLUSION: The low expression level of γ- synuclein inhuman ESCC and the biological effects of γ-synuclein over-expression on ESCC 9706 cells suggesting that γ-synuclein play play a role as a negative regulator in the development of human ESCC.