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目的目前，凋亡已成为肿瘤研究的热点之一，尤其基因对凋亡的调节作用已成为基因治疗和评价疗效的方向，其中以ｐ５３与ｂｃｌ－２基因尤为引人注目。因而，我们研究了鼻咽癌中凋亡与ｐ５３和ｂｃｌ－２基因表达的关系。材料与方法对近年我院治疗的９９例鼻咽癌病人的标本，通过免疫组化染色的方法测定突变型ｐ５３和ｂｃｌ－２基因的表达情况，并于光镜下根据形态学特征确定肿瘤凋亡细胞并计算其凋亡比例。结果我们发现ｐ５３表达－、＋、＋＋、＋＋＋的病理标本分别为３０、６０、４和５例，其凋亡指数为０．８３％、０．５５％、０．３７％及０．１４％，各组间统计有显著差异；８４例ｂｃｌ－２表达－、＋、＋＋、＋＋＋４组的病理标本为５７、１７、１０和０例，其凋亡揩数分别为０．６１％、０．４０％和０．３１％，各组间差异无显著性意义，但如将ｂｃｌ－２表达阳性两组“＋”、“＋＋”合并为阳性组后分析，则ｂｃｌ－２表达阴性与阳性之间差异有显著注意义（Ｐ＜０．０５）。同时将ｐ５３和ｂｃｌ－２基因表达分为３组：二者皆阴性，其中一者为阳性，二者皆为阳性组，其凋亡指数分别为０．８８％、０．５２％和０．４９％，双阳组与双阴组间差异有显著性意义（Ｐ＜０．０１）。结论鼻咽癌中?
Apoptosis has become one of the hot topics in cancer research. In particular, the role of genes in the regulation of apoptosis has become the direction of gene therapy and evaluation of curative effect. Among them, the p53 and bcl-2 genes are particularly attractive. Thus, we examined the relationship between apoptosis and p53 and bcl-2 gene expression in NPC. Materials and Methods The specimens of 99 NPC patients treated in our hospital in recent years were examined by immunohistochemical staining for the expression of mutant p53 and bcl-2 genes. The morphological features of the tumor were determined by light microscopy Dead cells and calculate the proportion of apoptosis. Results We found that the pathological specimens of p53 expression -, +, ++, +++ were 30, 60, 4 and 5, respectively. The apoptotic indexes were 0.83%, 0.55%, 0.37% and 0.14% There were significant differences among the groups. The pathological specimens of 84, bcl-2, +, ++ and +++ groups were 57, 17, 10 and 0, respectively. The number of apoptotic cells was 0.61% 40%, and 0.31% respectively. There was no significant difference between the groups. However, if the positive expression of bcl-2 positive group and positive group were combined, the expression of bcl-2 was negative and positive There was significant difference between the two groups (P <0.05). At the same time, p53 and bcl-2 gene expression was divided into three groups: both were negative, one was positive, both were positive group, the apoptosis index were 0.88%, 0.52% and 0 respectively. 49%, Shuangyang group and Shuangyin group differences were significant (P <0.01). Conclusion nasopharyngeal carcinoma?