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本文介绍一种由多种方法组合的技术，包括荧光素（FastBlae）逆行追踪．离体固定脑片细胞内LuciferYellow染色、荧光免疫组织化学染色和共聚焦激光扫描显微镜观察。通过这项组合技术．可以在1μm厚度的光学切片和连续光学切片三维重构图象中同时显示传出神经元的结构和免疫阳性传入纤维及其终未．进而可判断两种成分之间的联系。在单细胞水平的突触学研究中．此项技术提供了一个简单、有效的定性和初步定量相结合的实验方法．
This article describes a combination of techniques that include the FastBlae retrograde tracing. LuciferYellow staining, fluorescence immunohistochemical staining and confocal laser scanning microscopy were performed on the fixed intracerebral slices in vitro. Through this combination of technologies. Both the structure of the outgoing neurons and the immunopositive afferent fibers and their final expression can be simultaneously displayed in a 1 μm thick optical section and a continuous optical sectioned 3D reconstructed image. Which in turn can determine the link between the two components. In single cell level synaptic studies. This technique provides a simple and effective way to combine qualitative and preliminary quantitation.