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背景:学习和记忆的神经基础是中枢神经系统具有高度的可塑性,在中枢神经系统功能重组的过程中需要特定的康复训练。目的:观察运动训练对大脑中动脉梗死模型大鼠在实验中增强分辨学习能力和一次性被动回避反应的记忆保持能力的作用。设计:随机对照实验。单位:泸州医学院附属医院康复医学科。材料:实验于2002-07/2003-12在泸州医学院动物实验室完成。实验动物为8周龄Wistar雄性大鼠24只,随机分为脑梗死自由活动组(模型组)、脑梗死运动训练组(康复组)和正常组,每组8只。方法:①模型制备:康复组和模型组大鼠建立右侧大脑中动脉梗死模型,正常组不做处理。②运动训练:4d后对康复组大鼠进行4周的滚笼训练器、网屏训练器、平衡训练器训练,模型组和正常组不进行特殊的运动训练。③学习记忆测试:各组大鼠于术后第35天进行学习记忆行为学测试。Y型迷宫实验主要观察大鼠达到9/10正确反应(跑到暗臂)所需的训练次数;多功能条件反射箱实验主要观察大鼠在跳板上停留的时间(步入潜伏期)。主要观察指标:①各组大鼠达到常握迷宫结构标准所需的训练次数。②各组大鼠步入潜伏期。结果:24只大鼠均进入结果分析。①Y迷宫实验检测结果:康复组和正常组达到掌握迷宫结构标准所需的训练次数分别是(68.02±11.67)次和(57.62±10.31)次,而模型组则需要(107.07±16.32)次,模型组明显多于康复组和对照组(t=2.05,P<0.05)。②多功能条件反射箱实验结果:对大鼠一次性被动回避反应的记忆保持能力测试中显示,康复组和正常组步入潜伏期中位数分别是286.7s和298.4s,模型组为126.7s,模型组明显短于康复组和正常组(T=48,P<0.05)。结论:运动训练可以促进脑梗死大鼠学习记忆能力的恢复。
Background: The neural basis for learning and memory is the high degree of plasticity of the central nervous system that requires specific rehabilitation training in the functional reorganization of the central nervous system. OBJECTIVE: To observe the effects of exercise training on memory retention in experimental middle cerebral artery occlusion (MCAO) model to enhance discriminatory learning ability and one-time passive avoidance response. Design: Randomized controlled experiment. Unit: Luzhou Medical College Affiliated Hospital of Rehabilitation Medicine. Materials: The experiment was performed at Animal Laboratory of Luzhou Medical College from July 2002 to December 2003. Twenty-four Wistar male rats aged 8 weeks were randomly divided into free cerebral infarction group (model group), cerebral infarction training group (rehabilitation group) and normal group (n = 8). Methods: ①Model preparation: The right middle cerebral artery infarction model was established in rehabilitation group and model group, while the normal group was not treated. ② Exercise training: After 4 days, the rats in the rehabilitation group were trained for four weeks by using the cage trainer, the screen trainer and the balance trainer. The model group and the normal group did not perform any special exercise training. ③ learning and memory test: rats in each group on the 35th day after learning and memory behavioral tests. The Y-maze experiment mainly observed the number of training times required for rats to reach the 9/10 correct response (ran to the darkarm). The multi-purpose conditioned reflex box experiment mainly observed the time (step-in latency) of rats staying on the springboard. MAIN OUTCOME MEASURES: ① The number of training required to reach the standard of labyrinthine structure in each group of rats. ② rats into the incubation period. Results: Twenty-four rats were involved in the result analysis. (1) The results of Y maze test showed that the number of training required to control the labyrinthine structure of the rehabilitation group and the normal group was (68.02 ± 11.67) and (57.62 ± 10.31) times, respectively, while that of the model group was (107.07 ± 16.32) Group was significantly more than the rehabilitation group and the control group (t = 2.05, P <0.05). (2) The results of multi-functional conditional reflex box: The memory retention test of one-time passive avoidance reaction in rats showed that the median latency of walking into rehabilitation group and normal group were 286.7s and 298.4s respectively, the model group was 126.7s, The model group was significantly shorter than the rehabilitation group and the normal group (T = 48, P <0.05). Conclusion: Exercise training can promote the recovery of learning and memory ability in rats with cerebral infarction.