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材料来源经血清学检查及临床症状确诊为布鲁氏菌病(下称布病)患者100例(男70例,女30例)及健康人51例(男38例,女13例),年龄12~70岁,采静脉血肝素抗凝,于室温待查。淋巴细胞转化率(下称淋转)试验①淋巴细胞培养与制片:吸取肝素抗凝血0.2毫升种入已组合好的 RPMI1640培养基中(pH 7.2),加入
Materials Source 100 patients (70 males and 30 females) and 51 healthy people (38 males and 13 females) were diagnosed as having brucellosis (brucellosis) by means of serological tests and clinical symptoms. Age 12 to 70 years old, collecting venous blood heparin anticoagulant, to be investigated at room temperature. Lymphocyte transformation (hereinafter referred to as lymph node metastasis) Test ① Lymphocyte culture and preparation: draw 0.2 ml of heparin anticoagulant into the combined RPMI1640 medium (pH 7.2), add