本文采用纸层析比色法测定丹参注射液中有效成分——丹参素的含量,用10%氯化钠∶醋酸为展开剂,经纸层分离,碱性异菸肼显色后,斑点剪下洗脱,于495±3nm处测定丹参素的含量,符合比尔定律的浓度范围是0.4～2.0μg/ml(r=0.9994)。为评价异产地或同产地异批号的丹参注射液提供了一个准确、灵敏、快速的测定方法。 In this paper, paper chromatography colorimetry was used to determine the content of Danshensu, the active ingredient in Salvia miltiorrhiza injection. After 10% sodium chloride and acetic acid were used as the developer, the paper was separated and the basic isoniazid was developed. Under the elution, the content of danshensu was measured at 495±3 nm, and the concentration range according to Beer’s law was 0.4 to 2.0 μg/ml (r=0.9994). To provide an accurate, sensitive, and rapid method for evaluating Salvia miltiorrhiza injection that is from different origin or from the same place of origin.