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牙周炎症的牙龈组织中存在的超氧离子可引起细胞 DNA 单链断裂,正常细胞 DNA 单链断裂能及时修复,如果大量 DNA 单链断裂不能很快修复,则可导致局部基因表达和调节异常。作者对牙周炎症部位和健康部位成纤维细胞 DNA 单链断裂状况,DNA 损伤修复的重要酶——DNA 连接酶和多聚腺嘌呤二磷酸核糖(PADPR)合成酶的活性进行了研究,结果表明,牙周炎症部位牙龈成纤维细胞的 DNA 单链断裂数目明显高于健康部位者(P<0.01);其 DNA 连接酶活性及 PADPR 合成酶活性均显著低于健康部位的酶活性(P<0.01);作者对实验结果的可能机制进行了分析讨论。
Superoxide ions present in periodontal inflammatory gingival tissue can cause single-stranded DNA breaks in cells and single-stranded DNA breaks in normal cells to be repaired in time, leading to abnormal local gene expression and modulation if large numbers of single-stranded DNA breaks can not be rapidly repaired . The authors investigated DNA single-strand breaks of fibroblasts at periodontal inflammatory sites and healthy parts, DNA ligase and PADPR synthetase activity of DNA damage repair, the results showed (P <0.01). The DNA ligase activity and PADPR synthase activity in gingival fibroblasts at periodontal inflammation sites were significantly higher than those at healthy sites (P <0.01) ); The author analyzes the possible mechanism of experimental results.