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目的:探讨小泛素样修饰蛋白(SUMO)化修饰对核因子(NF)-κB通路激活的负性调控在2型糖尿病大鼠脑部病变中的干预作用,为前列地尔对其病变的早期干预提供依据。方法:随机抽取40周龄的10只GK大鼠为前列地尔组(前列地尔2.5μg/g,尾尖静脉注射给药,1次/d,共10d)、10只为糖尿病对照组,另取10只Wistar雄性大鼠作为正常对照组。正常对照组和糖尿病对照组给予等剂量生理盐水,均为尾尖静脉注射给药。三组均给予普通饲料。测定和观察血糖、甘油三酯(TG)、总胆固醇(TC)变化及NF-κB抑制因子(IκB)、NF-κB、SUMO1的水平变化。结果:(1)糖尿病对照组、前列地尔组大鼠的血糖、TG、TC均显著高于正常对照组(P均<0.05);(2)HE染色观察:正常对照组脑组织细胞形态正常,糖尿病对照组、前列地尔组脑组织可见细胞肥大,疏松样改变,前列地尔组病变较轻;(3)免疫组化测定的IκB、NF-κB、SUMO1:正常对照组少许阳性表达,糖尿病对照组、前列地尔组表达均显著增加(P<0.05或<0.01),但前列地尔组较糖尿病对照组IκB[(0.242±0.034)比(0.268±0.017)]、NF-κB[(0.373±0.015)比(0.486±0.013)]表达显著减少,SUMO1[(0.463±0.015)比(0.343±0.018)]表达显著增加(P均<0.05)。结论:小泛素样修饰蛋白在糖尿病大鼠脑组织表达增多,在前列地尔组表达更多,前列地尔对糖尿病大鼠脑组织病变有一定改善作用。
Objective: To investigate the effect of SUMOylation on the regulation of nuclear factor (NF) -κB pathway activation in brain lesions of type 2 diabetic rats, and to investigate the effects of alopecia areata Provide the basis for early intervention. Methods: Ten 40-week-old GK rats were randomly selected as the alprostadil group (alprostadil 2.5μg / g, tail vein injection once, d for 10 days) Another 10 Wistar male rats as normal control group. Normal control group and diabetic control group were given equal doses of saline, are tail vein injection. Three groups were given ordinary feed. The changes of blood glucose, triglyceride (TG), total cholesterol (TC) and the levels of NF-κB inhibitor, IκB, NF-κB and SUMO1 were measured and observed. Results: (1) The blood glucose, TG and TC of diabetic control group and alprostadil group were significantly higher than those of normal control group (all P <0.05). (2) HE staining showed that normal control group had normal morphology of brain cells , Diabetes mellitus group and alprostadil group, the cell hypertrophy and looseness were observed in the brain tissue of the alprostadil group, and the lesion in the alprostadil group was lighter; (3) IκB, NF-κB and SUMO1 in immunohistochemistry were slightly positive in the normal control group, (P <0.05 or <0.01) in the diabetic control group and the alprostadil group, but there was no difference between the alprostadil group and the diabetic control group (0.268 ± 0.017 vs 0.248 ± 0.034, 0.373 ± 0.015) (0.486 ± 0.013)], and the expression of SUMO1 was significantly increased in [(0.463 ± 0.015) vs (0.343 ± 0.018)] (all P <0.05). Conclusion: The expression of small ubiquitin - like modified protein in the brain tissue of diabetic rats increased, and more in the alprostadil group, alprostadil on diabetic rat brain tissue lesions have some improvement.