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目的探讨β-双酮类抗生素对水生鱼类斑马鱼的毒性作用及其机制。方法将斑马鱼胚胎暴露于一定浓度的诺氟沙星和四环素溶液中,观察毒液对斑马鱼胚胎的影响;21 d毒性试验后用RT-PCR法检测斑马鱼内分泌系统和免疫系统相关基因的mRNA水平。结果在胚胎发育过程中其死亡率、畸形率和暴露浓度息息相关,随着浓度升高(>50 mg/L),孵化率逐渐降低,死亡率、总畸形率逐渐升高;幼鱼发育畸形主要表现为心包囊水肿、卵黄囊水肿等;盐酸四环素暴露引起斑马鱼VTG1、IL-1β和TNK-α的mRNA的水平升高,且与剂量呈正相关,CYP 1A表达量稍微上升,与对照组差异无统计学意义。结论斑马鱼内分泌系统和免疫系统相关基因VTG1、IL-1β和TNK-α对β-双酮类抗生素暴露较敏感,有望成为生物标志物,有效评价生态环境。
Objective To investigate the toxic effects of β-diketone on zebrafish and its mechanism. Methods The zebrafish embryos were exposed to a concentration of norfloxacin and tetracycline solution to observe the effects of venom on zebrafish embryos. After 21 days’ toxicity test, mRNA of zebrafish endocrine system and immune system related genes was detected by RT-PCR Level. Results During embryonic development, the mortality, deformity rate and exposure concentration were closely related. With the increase of concentration (> 50 mg / L), the hatching rate gradually decreased and the mortality rate and total deformity rate gradually increased. Cytoplasmic edema and yolk sac edema. The mRNA levels of zebrafish VTG1, IL-1β and TNK-α were increased after exposure to tetracycline hydrochloride, and positively correlated with the dose. The expression of CYP 1A increased slightly, which was significantly different from the control group No statistical significance. Conclusion The zebrafish endocrine system and the immune system related genes VTG1, IL-1β and TNK-α are sensitive to the exposure of β-diketone antibiotics and are expected to become biomarkers to evaluate the ecological environment effectively.