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目的:检测系统性红斑狼疮(systemic lupus erythematosus,SLE)患者血清中CD83(soluble CD 83,sCD 83)和多种自身抗体的表达水平,并探讨其相互关系。方法:ELISA检测患者可溶性CD 83和AnuA的表达,应用间接免疫荧光的方法检测抗cmDNA抗体,应用乳凝法检测血清中的DNP,采用胶体金标记和快速膜渗滤技术测定血清中的抗dsDNA抗体。结果:对照组患者血清中可溶性CD 83的表达为(0.26±0.10)ng/ml,实验组患者血清中可溶性CD 83的表达为(5.56±0.72)ng/ml。与对照组相比,实验组患者血清中可溶性CD 83的平均浓度明显升高。在抗dsDNA抗体阴性的51例系统性红斑狼疮患者中AnuA的阳性率明显高于抗DNP抗体和抗cmDNA抗体,同样在抗DNP抗体阴性的58例系统性红斑狼疮患者中AnuA的阳性率明显高于dsDNA抗体和抗cmDNA抗体。系统性红斑狼疮患者中可溶性CD83的水平(<2.68 ng/ml)与各种自身抗体(抗dsDNA抗体、AnuA、抗DNP抗体和抗cmDNA抗体)水平的相关系数分别为(r=0.542,0.613,0.489和0.367)。具有高水平可溶性CD83的系统性红斑狼疮患者(≥2.68 ng/ml),与各种自身抗体(抗dsDNA抗体,AnuA,抗DNP抗体和抗cmDNA抗体)水平的相关系数分别为(r=0.711,P<0.05)、(r=0.845,P<0.01)、(r=0.862,P<0.01)和(r=0.724,P<0.051)。结论:可溶性CD83通过活化DC细胞并激活补体系统,参与系统性红斑狼疮的发生发展,联合可溶性CD83和多种自身抗体的检测,能更明确系统性红斑狼疮患者病情的严重程度,有利于SLE的诊断和治疗。
OBJECTIVE: To detect the expression of CD83 (sCD83) and multiple autoantibodies in sera of patients with systemic lupus erythematosus (SLE) and to explore their relationship. Methods: The expression of soluble CD 83 and AnuA in patients was detected by ELISA. The anti-cmDNA antibody was detected by indirect immunofluorescence. The DNP in serum was detected by the method of emulsion coagulation. The anti-dsDNA in serum was detected by colloidal gold labeling and rapid membrane diafiltration antibody. Results: Serum soluble CD 83 expression was (0.26 ± 0.10) ng / ml in the control group and 5.56 ± 0.72 ng / ml in the experimental group. Compared with the control group, the mean concentration of soluble CD 83 in the serum of the experimental group was significantly increased. The positive rate of AnuA in 51 cases of systemic lupus erythematosus patients with anti-dsDNA antibody was significantly higher than that of anti-DNP antibody and anti-cmDNA antibody. Also, the positive rate of AnuA in 58 cases of systemic lupus erythematosus patients with negative DNP antibody was significantly higher DsDNA and anti-cmDNA antibodies. The correlation coefficients of soluble CD83 levels (<2.68 ng / ml) and levels of various autoantibodies (anti-dsDNA, AnuA, anti-DNP and anti-cmDNA) in patients with systemic lupus erythematosus were (r = 0.542,0.613, 0.489 and 0.367). Correlation coefficients with the levels of various autoantibodies (anti-dsDNA, AnuA, anti-DNP and anti-cmDNA) in patients with systemic lupus erythematosus (≥2.68 ng / ml) with high levels of soluble CD83 were (r = 0.711, (R = 0.845, P <0.01), (r = 0.862, P <0.01) and (r = 0.724, P <0.05). Conclusion: Soluble CD83 is involved in the pathogenesis of systemic lupus erythematosus by activating DCs and activating complement cells. Combined with the detection of soluble CD83 and multiple autoantibodies, soluble CD83 can clarify the severity of SLE patients and benefit SLE patients Diagnosis and treatment.