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应用绝对分析方法在高效波相色谱上对芳香胺类化合物的定量可行性进行了研究。用紫外分光光度计测定芳香胺类化合物的摩尔吸光系数,并以此为标准探讨用绝对分析法对芳香胺的定量准确性,并对定量误差的原因进行了讨论。同时,在不同配比的甲醇-磷酸盐缓冲体系为流动相时,应用绝对分析法对芳香胺摩尔吸光系数的变化进行了研究,结果发现芳香胺的摩尔吸光系数随流动相中甲醇的比例的变化而变化很大。
The feasibility of quantitative analysis of aromatic amines was studied by using absolute analytical method in high performance liquid chromatography. The molar absorptivity of aromatic amines was determined by UV spectrophotometer. The quantitative accuracy of aromatic amines by absolute analysis was discussed. The reason of the quantitative error was also discussed. At the same time, when different ratios of methanol-phosphate buffer system were used as mobile phase, the change of molar absorptivities of aromatic amines were studied by absolute analysis. The results showed that the molar absorptivity of aromatic amines with the ratio of methanol in mobile phase Changes and changes vary greatly.