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目的探讨TCF7L2基因rs10885410、rs7100927位点和IDE基因rs10882066、rs11187007、rs7078413位点单核苷酸多态性(SNP)与妊娠期糖尿病(GDM)遗传易感性的关系。方法选取2011年9月至2013年8月在中国医科大学附属盛京医院住院分娩的GDM孕妇178例为病例组,同期121例葡萄糖耐量正常的妊娠妇女作为对照组。提取受试者全基因组DNA,对TCF7L2基因rs10885410、rs7100927位点和IDE基因rs10882066、rs11187007、rs7078413位点进行基因分型,并进行相关分析。结果 TCF7L2基因rs10885410、rs7100927位点各基因型和等位基因频率分布,两组间差异无统计学意义(P>0.05)。IDE基因的rs11187007位点,病例组AA、AG、GG 3种基因型频率分别为4.49%、56.18%、39.33%,对照组分别为10.74%、38.84%、50.42%,两组比较,P<0.05。AG基因型GDM的发病风险与AA基因型相比,OR值为3.457(95%CI 1.342~8.909,P=0.010),经Logistic回归校正混杂因素后,OR值为3.403(95%CI 1.072~10.803,P=0.038)。结论 IDE基因的rs11187007位点多态性可能与GDM遗传易感性有关;AG基因型可能是其发生的危险性因素。
Objective To investigate the relationship between genetic polymorphisms of rs10885410, rs7100927 and IDE gene rs10882066, rs11187007 and rs7078413 of TCF7L2 gene and genetic susceptibility to gestational diabetes mellitus (GDM). Methods Seventy-eight pregnant women with GDM hospitalized in Shengjing Hospital Affiliated to China Medical University from September 2011 to August 2013 were selected as the case group and 121 pregnant women with normal glucose tolerance as the control group. The complete genomic DNA of the subjects was extracted and the genotypes of rs10885410, rs7100927 and IDE gene rs10882066, rs11187007 and rs7078413 of TCF7L2 gene were genotyped and analyzed. Results There was no significant difference in genotype and allele frequency between TCF7L2 rs10885410 and rs7100927 (P> 0.05). IDE gene rs11187007 locus, the cases of AA, AG, GG genotype frequencies were 4.49%, 56.18%, 39.33%, respectively, the control group were 10.74%, 38.84%, 50.42%, P <0.05 . The risk of AG genotype GDM was 3.457 (95% CI 1.342-8.909, P = 0.010) compared with AA genotype. After adjusting for confounding factors by Logistic regression, OR was 3.403 (95% CI 1.072-10.803 , P = 0.038). Conclusion The rs11187007 polymorphism of IDE gene may be related to genetic susceptibility to GDM. The AG genotype may be a risk factor for its occurrence.