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为了提高Folin-Ciocalteu比色法的选择性,以邻苯二酚作为多酚模型化合物,建立了植物多酚固相萃取方法。自制17种交联聚合物,从聚合物组成的库中筛选出对邻苯二酚吸附最好的聚合物,即衣康酸和乙二醇二甲基丙烯酸酯的交联聚合物(IA-co-EDMA),聚合物对邻苯二酚的表观吸附量达到41.60 mg/g。以IA-co-EDMA为固相萃取剂,进行萃取并条件优化,用0.1 mol/L HCl溶液上样、水淋洗、体积比95:5的甲醇水洗脱时,没食子酸的萃取回收率达到99.5%。结合固相萃取,通过Folin-Ciocalteus比色法测定玫瑰花中的总多酚含量为92.23 mg/g,加标回收率为98.2%～101.3%(RSD为2.3%～4.7%)。对抗坏血酸加标萃取液进行固相萃取,上样和淋洗过程全部被排除。方法能够消除抗坏血酸对Folin-Ciocalteus法测定多酚的干扰,可用于高选择性和准确测定植物多酚。
In order to improve the selectivity of the Folin-Ciocalteu colorimetric method, a catechol was used as a polyphenol model compound to establish a plant polyphenol extraction method. Seventeen kinds of cross-linked polymers were self-made, and the polymers which were best adsorbed on catechol were selected from the polymer library. The cross-linked polymers of itaconic acid and ethylene glycol dimethacrylate (IA- co-EDMA), the apparent adsorption capacity of the polymer to catechol reached 41.60 mg / g. With IA-co-EDMA as solid-phase extraction solvent, extraction and optimization of conditions, with 0.1 mol / L HCl solution loading, water rinse, the volume ratio of 95: 5 methanol water elution, gallic acid extraction recovery Reached 99.5%. Combined with solid phase extraction, the total polyphenol content of rose was 92.23 mg / g as determined by Folin-Ciocalteus colorimetric method, and the recoveries were 98.2% -101.3% (RSD 2.3% -4.7%). Ascorbic acid spiked extract for solid phase extraction, loading and rinsing process were all excluded. The method can eliminate the interference of ascorbic acid on the determination of polyphenols by Folin-Ciocalteus method and can be used for the determination of plant polyphenols with high selectivity and accuracy.