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西花蓟马Frankliniella occidentalis(Pergande)是一种世界性入侵害虫,寄主范围广,危害严重,2003年首次在我国发生危害,并有进一步扩散蔓延的趋势。针对蓟马类害虫虫体微小、形态相似,难以准确快速区分的问题,采用特征序列扩增区域(SCAR)标记技术,以西花蓟马及与之同域发生的其他种类蓟马为对象,筛选出1对西花蓟马特异性引物(FOMF/FOMR),其扩增片段大小为320bp。种特异性检测结果显示,该对引物只对西花蓟马的基因组DNA具有扩增能力,对同域发生的花蓟马F.intonsa(Trybom)、禾花蓟马F.tenuicornis(Uzel)、烟蓟马Thripstabaci L.等41种蓟马不具有扩增效果。该对引物不仅对不同虫态的西花蓟马具有扩增能力,而且在西花蓟马发生地的寄主植物组织内亦检测到了其卵的存在。同时,该检测技术灵敏度高,对成虫的最低检出阈值为1/160头。本检测技术在口岸检疫以及花卉、蔬菜和种苗调运中的害虫检测和监测中具有重要意义。
Frankliniella occidentalis (Pergande), a world-wide invasive pest, has a wide range of hosts and serious harm. It was the first time that harm occurred in China in 2003, and there is a tendency of spreading and spreading further. Aiming at the problem that thrips pest insects are tiny, the morphology is similar and it is difficult to distinguish accurately and rapidly, SCAR markers are used to identify the species of thrips A pair of western flower thrips specific primers (FOMF / FOMR), the amplified fragment size of 320bp. Species-specific test results showed that the pair of primers only had the ability to amplify the genomic DNA of F. occidentalis, and the homology of F.intonsa (Trybom), F. tenuicornis (Uzel) 41 thrips such as Thripstabaci L. did not have amplification effects. The pair of primers not only have the ability to amplify the different Wilt F. occidentalis, but also detect the presence of their eggs in the host plant tissue of the western flower thrips. At the same time, the detection technique has high sensitivity and the minimum detection threshold for adults is 1/160. The detection technology is of great significance in the quarantine of ports and the detection and monitoring of pests in flowers, vegetables and seedlings.