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体外测定表明,木霉菌株T—169和T—279对病原菌Rhizoctoniasoclani(立枯丝核菌)、Fusariumoxysporum(尖孢镰刀菌)、Pythiumaphamidermatum(腐霉)和Alternariatenuis(交链孢霉)在对峙培养中的拮抗系数分别为2、2~3和3。温室测定表明,用0.6%(W/W)T-169麸皮培养物(107cfu/g)处理土壤。在人工接种立枯丝核菌、尖孢镰刀菌、腐霉及交链孢霉20d后,落叶松苗木发病率分别比未用木霉处理的对照减少49.6%、17.4%、18.3%和11.7%;用T-169和T-279木霉孢子悬浮液(108cfu/ml)处理落叶松种子,人工接种立枯丝核菌19d后,落叶松成苗率分别比未用木霉处理的对照增加18.8%和12.7%。
In vitro assays showed that Trichoderma strains T-169 and T-279 were resistant to the pathogenic bacteria Rhizoctonia solani, Fusarium oxysporum, Pythiuma phamidermatum and Alternaria alternans The antagonistic coefficients were 2,2 ~ 3 and 3, respectively. Greenhouse assays showed that the soil was treated with 0.6% (W / W) T-169 wheat bran culture (107 cfu / g). After artificial inoculation of Rhizoctonia solani, Fusarium oxysporum, Pythium sp. And Alternaria sp. For 20 days, the incidence of larch seedlings was reduced by 49.6%, 17.4% and 18.4% respectively compared with the control without Trichoderma treatment .3%, and 11.7% respectively. Larix seeds were treated with T-169 and T-279 Trichoderma sp. Suspension (108 cfu / ml) Controls treated with Trichoderma increased 18.8% and 12.7%.