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目的了解漳州市海、水产品中致病性弧菌的污染状况及其菌株的耐药性。方法采集市场、餐厅销售的海、水产品,取其腮部、肠容物或去壳取肉,分别接种于碱性和3.5%NaCl蛋白胨水中进行增菌,碱性蛋白胨水增菌液划线接种庆大琼脂平板,3.5%NaCl蛋白胨水增菌液划线接种TCBS琼脂平板分离培养。对分离菌进行系统生化和血清学分型鉴定;常规PCR检测霍乱弧菌肠毒素ctxA基因,K-B法作药敏试验。结果 450份海、水产品中,致病性弧菌检出率为58.9%,其中非O1群霍乱弧菌87.9%、O1群霍乱弧菌7.9%、霍利斯弧菌1.9%、溶藻弧菌1.1%、副溶血性弧菌0.8%、河弧菌0.4%。265株致病性弧菌对抗菌药物阿莫西林、环丙沙星、头孢呋新、头孢噻肟、头孢唑林、复方新诺明的敏感性均在95%以上,但对氯霉素、氨苄西林、四环素有不同程度的耐药性。结论漳州市海、水产品中存在6种致病性弧菌污染,对其进行药敏监测,可为预防、治疗用药提供参考。
Objective To understand the pollution status of pathogenic Vibrio in Zhangzhou sea and aquatic products and the drug resistance of the strains. Methods Seawater and aquatic products sold in the market and restaurant were collected, and their cheeks, intestinal contents or shelled meat were taken and inoculated into alkaline and 3.5% NaCl peptone water respectively for enrichment. The alkaline peptone water added broth was scribed Inoculation Qingda agar plate, 3.5% NaCl peptone water broth by line inoculation TCBS agar plate separation and culture. Systematic biochemical and serological typing of the isolates were carried out. The conventional PCR was used to detect the ctxA gene of V. cholerae enterotoxin. K-B method was used for drug sensitivity test. Results Among 450 sea and fishery products, the detection rate of pathogenic Vibrio was 58.9%, of which Vibrio cholera non-O1 group 87.9%, Vibrio cholerae O1 group 7.9%, Hollis Vibrio 1.9% 1.1% of bacteria, 0.8% of Vibrio parahaemolyticus, 0.4% of Vibrio cholerae. 265 strains of pathogenic Vibrio were more than 95% sensitive to the antibacterial drugs amoxicillin, ciprofloxacin, cefuroxime, cefotaxime, cefazolin, cotrimoxazole, but the chloramphenicol, Ampicillin, tetracycline have different levels of resistance. Conclusion There are 6 kinds of pathogenic vibrio contamination in the sea and aquatic products in Zhangzhou City, and their drug susceptibility monitoring can provide a reference for the prevention and treatment of drug use.