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目的:探讨信号转导和转录激活子3(STAT-3)及其相关转录因子在新生鼠巨细胞病毒(MCMV)感染中的表达变化及其意义。方法:48只新生BALB/c小鼠按窝随机分为病毒组(n=24)和对照组(n=24)。两组动物均设3天、7天、14天3个时间点亚组,各亚组平均8只小鼠。病毒组于仔鼠出生3天时腹腔接种致半数细胞感染量为104.31μ/0.1 ml的MCMV病毒悬液20μl,建立MCMV播散性感染模型;对照组腹腔注射等量的无菌生理盐水。各组于规定时间点处死仔鼠,HE染色观察仔鼠肝、脾、肺组织病理改变;半定量RT-PCR法检测脾脏STAT-3mRNA、孤独核受体(RORγt)mRNA及白介素-17(IL-17)mRNA的表达。结果:1病毒组小鼠肝、脾、肺组织MCMV-DNA PCR检测出现阳性条带,HE染色出现相应病理改变。2病毒组小鼠脾组织STAT-3mRNA、RORγtmRNA及IL-17 mRNA的表达水平在感染后7天、14天明显高于对照组(P<0.01)。结论:STAT-3及其相关因子的高表达可能促进巨细胞病毒在体内的复制,为巨细胞病毒感染的发病机制之一。
OBJECTIVE: To investigate the changes of signal transducer and activator of transcription 3 (STAT-3) and its related transcription factors in neonatal murine cytomegalovirus (MCMV) infection and its significance. Methods: 48 neonatal BALB / c mice were randomly divided into virus group (n = 24) and control group (n = 24). Two groups of animals were set 3 days, 7 days, 14 days at 3 time points subgroup, an average of 8 mice in each subgroup. The virus group was intraperitoneally inoculated intraperitoneally with 20μl of MCMV virus suspension which caused a half cell infection of 104.31μ / 0.1 ml on the 3rd day of birth, and the control group was given the same amount of sterile saline. The pups were sacrificed at the prescribed time points, and the pathological changes of the liver, spleen and lungs were observed by HE staining. The expressions of STAT-3 mRNA, RORγt mRNA and interleukin-17 -17) mRNA expression. RESULTS: Positive bands were detected by PCR with MCMV-DNA in the liver, spleen and lung of 1 virus group, and the corresponding pathological changes were observed by HE staining. The expression levels of STAT-3mRNA, RORγt mRNA and IL-17 mRNA in spleen tissues of 2 virus-infected mice were significantly higher than that of the control group at 7 and 14 days after infection (P <0.01). Conclusion: The high expression of STAT-3 and its related factors may promote cytomegalovirus replication in vivo, which is one of the pathogenesis of cytomegalovirus infection.