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为研究高效氯氰菊酯亚致死浓度对桃小食心虫Carposina sasakii Matsumura雌、雄蛾体内解毒酶活性的影响,采用药膜法,以LC_10、LC_20和LC_40浓度的高效氯氰菊酯处理桃小食心虫成虫24 h,分别测定药剂压力解除后0、6、12和24 h桃小食心虫雌、雄蛾体内细胞色素P450单加氧酶7-乙氧基香豆素-O-脱乙基酶(ECOD)、羧酸酯酶(CarE)和谷胱甘肽S-转移酶(GSTs)的活性,并分析其动态变化。结果表明:与对照相比,高效氯氰菊酯LC_10浓度处理能抑制桃小食心虫成虫的ECOD比活力,且随处理浓度增加,高效氯氰菊酯对ECOD活性的诱导作用逐渐体现,LC_40浓度处理组ECOD活性显著高于对照;此外,LC_20和LC_40浓度处理组可诱导桃小食心虫雄蛾CarE和GSTs比活力的增加,但对于雌蛾则结果相反。当药剂压力解除后,随着处理时间的延长,成虫体内ECOD、CarE和GSTs活性总体表现为先增加后降低,而药剂浓度越高对其解毒酶的诱导或抑制作用越显著,且存在一定性别差异。研究显示,从短期响应来看,桃小食心虫雌、雄蛾体内的3种解毒酶在高效氯氰菊酯亚致死浓度胁迫后均表现较为活跃,酶活性呈现出先增加后降低的动态变化。由于雌、雄蛾个体的生理机能以及对药剂敏感性的差异,这种生理响应机制存在一定的剂量和性别差异。
In order to study the effects of beta-cypermethrin on detoxification enzyme activity in female and male moths Carposina sasakii Matsumura, the adults were treated with alpha-cypermethrin (LC_10, LC_20 and LC_40) at 24, The release of 7-ethoxycoumarin-O-deethylase (ECOD), carboxylesterase (CarE) and glutathione S-transferase (GSTs) activity, and analyze its dynamic changes. The results showed that compared with the control, the concentration of cypermethrin LC_10 could inhibit the ECOD activity of adult Beauveria bassiana. With the increase of the treatment concentration, the effect of the beta-cypermethrin on the ECOD activity gradually appeared. The activity of ECOD in the LC_40 group was significantly higher than that of the control In addition, the LC_20 and LC_40 concentration-treated groups could induce the increase of specific activities of CarE and GSTs in male peach moth, but the opposite results were observed in female moths. When the drug pressure was relieved, the activity of ECOD, CarE and GSTs in the adult showed the first increase and then decreased with the prolongation of treatment time, while the higher the concentration of the drug was, the more significant the induction or inhibition of detoxification enzyme and the existence of certain gender difference. The results showed that three kinds of detoxification enzymes in female and male moths were more active after sub-lethal concentration of beta-cypermethrin, and the enzyme activity showed the first increase and then the decrease in the dynamic response. Due to the physiological function of the individual female and male, as well as the sensitivity of the drug differences, this physiological response mechanism there is a certain dose and gender differences.