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目的：探究蛋白激酶 CK2表达下调对血管内皮细胞形成新生血管的能力以及对其细胞中血管内皮生长因子（VEGF）表达的影响。方法化学合成 siRNA 转染人脐静脉内皮细胞（HUVECs），用 RT-PCR 法和 Western blotting 法检测细胞转染效率；MTT 法检测 CK2表达下调对 HUVECs 细胞增殖的影响；成管实验和划痕实验检测CK2表达下调对细胞成管、迁移的影响；Real-time PCR 和 Western blotting 法检测 siRNA 干扰蛋白激酶 CK2表达后 VEGF mRNA 及蛋白的表达。结果siRNA干扰蛋白激酶 CK2达到了预期干扰率75％，蛋白激酶 CK2表达下调后抑制了 HUVECs 增殖、成管、迁移（P ＜0．05），也抑制了 VEGF mRNA 及蛋白的表达。结论蛋白激酶 CK2表达下调可抑制 VEGF 的表达及 HUVECs 的增殖、成管、迁移。“,”Objective To prove the effect of Casein Kinase2 siRNA on vascular endothelial growth factor(VEGF) expression and the angiogenic capacity in human umbilical vein endothelial cells (HUVECs).Methods HUVECs were transfected with either Casein Kinase2 siRNA or negative control siRNA.Cell proliferation was analyzed by MTT assay;the angiogenic capacity was accessed by tube formation;cell migration was assessed by the wound-healing mod-el.Gene and protein expressions of VEGF were examined by Real-time PCR and Western blotting methods.Results Casein Kinase2 siRNA decreased the expressions of Casein Kinase2 and declined HUVEC proliferation,tube formation and migration.It was also reduced the expression of VEGF mRNA and VEGF protein.Conclusion Our results dem-onstrated that Casein Kinase2 siRNA reduced the expression of VEGF and declined the capacity of angiogenesis in HU-VECs.