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该文以优系短枝富士为主要试材，采用木本指示植物和间接酶联免疫（ＰＡＳ－ＥＬＩＳＡ）两种方法，对苹果褪绿叶斑病毒（ＣＬＳＶ）、苹果茎沟病毒（ＳＧＶ）和苹果茎痘病毒（ＳＰＶ）进行了检测．试验表明，俄罗斯苹果Ｒ１２７４０－７Ａ、司派２２７和弗吉尼亚小苹果３种指示植物可作为上述３种潜在病毒的鉴定植物．植物生长状况对鉴定结果有重大影响．间接酶联免疫检测速度快，准确度高．该试验所用最佳抗体工作浓度ＣＬＳＶ：第一抗体１／６０００，第二抗体１／２０００；ＳＧＶ：第一抗体１／５０００，第二抗体１／３０００．
In this paper, we studied the effects of CLSV, SGV and PSV by using two methods of woody plants and indirect enzyme-linked immunosorbent assay (ELISA) Apple stem pox virus (SPV) was tested. Tests showed that three indica plants of Russian apple R12740-7A, sesame 227 and Virginia small apple can be used as the identification plants of the three potential viruses mentioned above. Plant growth status has a significant impact on the identification results. Indirect ELISA detection speed, high accuracy. The best antibody working concentration used in this experiment CLSV: the first antibody 1/6000, the second antibody 1/2000; SGV: the first antibody 1/5000, the second antibody 1/3000.