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目的:克隆人IL-37b基因cDNA,并构建其真核表达载体。方法:从包皮组织中提取总RNA,用逆转录-PCR技术,扩增人IL-37b基因全长编码区片段,经酶切后,克隆入pcDNA3.1(+)载体中,构建真核表达载体pcDNA3.1-hIL-37b,用内切酶双酶切鉴定与DNA测序。结果:获得的IL-37b基因序列与GenBank数据库中的一致。人IL-37b基因的全长编码片段为657 bp,翻译为218个氨基酸。结论:人IL-37b基因成功地克隆并构建了其真核表达载体,为进一步进行IL-37的表达与功能研究奠定了基础。
Objective: To clone human IL-37b cDNA and construct its eukaryotic expression vector. Methods: The total RNA was extracted from foreskin tissue and the full-length coding region of human IL-37b gene was amplified by RT-PCR. After digestion, the recombinant plasmid was cloned into pcDNA3.1 (+) vector to construct eukaryotic expression vector Vector pcDNA3.1-hIL-37b, double digestion with restriction endonucleases and DNA sequencing. Results: The obtained IL-37b gene sequence is consistent with the GenBank database. The full-length coding fragment of human IL-37b gene is 657 bp, translated into 218 amino acids. CONCLUSION: Human IL-37b gene was successfully cloned and constructed its eukaryotic expression vector, which laid the foundation for the further study of IL-37 expression and function.